Simple Plex Human Angiopoietin-like Protein 4 Cartridge

Catalog #: SPCKB-PS-000291 Datasheet
Simple Plex Human Angiopoietin-like Protein 4/ANGPTL4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000291
SPCKA-PS-000291
SPCKC-PS-000291
Control Products Available
Ella Automated ELISA Platform and Simple Plex Human Angiopoietin-like 4 Assay
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Simple Plex Human Angiopoietin-like Protein 4 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity
93.2 pg/mL
Assay Range
546.0 - 333,200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Angiopoietin-like 4.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Angiopoietin-like 4 in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

Recovery

Recovery was not necessary as human Angiopoietin-like 4 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Angiopoietin-like 4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Simple Plex Human Angiopoietin-like 4 Assay Linearity

Scientific Data

Simple Plex Human Angiopoietin-like 4 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Angiopoietin-like 4 Assay. Assay range is 546-333,200 pg/mL.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:
51129 (Human); 57875 (Mouse); 362850 (Rat)
Alternate Names:
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein

FAQs

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