Simple Plex Discovery Human Uteroglobin Assay

Catalog #: SPCKB-PS-012701 Datasheet
Simple Plex Human Uteroglobin assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-012701
SPCKA-PS-012701
SPCKC-PS-012701
Control Products Available
Simple Plex Discovery Human Uteroglobin Ella Assay
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Simple Plex Discovery Human Uteroglobin Assay Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
5.03 pg/mL
Assay Range
8.0 - 1,954 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Uteroglobin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Uteroglobin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 25 25
Mean (pg/mL) 18.3 839 17.8 868
Standard Deviation 1.19 29.5 1.91 74.9
CV% 6.5 3.5 10.7 8.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 106 106-106

Linearity

Samples containing and/or spiked with high concentrations of human Uteroglobin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Simple Plex Discovery Human Uteroglobin Ella Assay Linearity

Scientific Data

Simple Plex Discovery Human Uteroglobin Ella Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Uteroglobin/SCGB1A1

Uteroglobin is small, secreted protein of the secretoglobin (SCGBs) family. Uteroglobin is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia that communicate with the external environment. Uteroglob in binds to phospholipids and to several cell types, inhibiting inflammation, migration and invasion. It has been reported to bind the lipocalin-1 receptor.

Entrez Gene IDs:
7356 (Human); 22287 (Mouse); 25575 (Rat)
Alternate Names:
Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin

FAQs

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