Simple Plex Cytokine Storm Panel
Simple Plex Cytokine Storm Panel Summary
The Simple Plex Human Multianalyte Assay is for the detection of human IL-1 beta, IL-6 3rd gen, IL-8, and TNF-alpha in serum and plasma (EDTA/Heparin).| Sample Type & Volume Required Per Well | Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL) |
| Sensitivity | IL-1 beta 0.16 pg/mL; IL-6 3rd gen 0.11 pg/mL; IL-8 0.19 pg/mL; TNF-alpha 0.3 pg/mL |
| Assay Range | IL-1 beta 0.16-1,530 pg/mL; IL-6 3rd gen 0.28-2,652 pg/mL; IL-8 0.19-1,804 pg/mL; TNF-alpha 0.3-1,160 pg/mL |
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human IL-1 beta/IL-1F2, IL-6 3rd gen, IL-8/CXCL8, and TNF alpha. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| IL-1 beta/IL-1F2 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 8.66 | 446 | 8.42 | 410 | |
| Standard Deviation | 0.427 | 16.5 | 0.342 | 23.3 | |
| CV (%) | 4.9 | 3.7 | 4 | 5.7 | |
| IL-6 3rd gen | n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 23.1 | 876 | 23.6 | 1008 | |
| Standard Deviation | 0.899 | 72.1 | 1.82 | 77.7 | |
| CV (%) | 3.9 | 8.2 | 7.7 | 7.7 | |
| IL-8/CXCL8 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 28.7 | 1362 | 27.3 | 1463 | |
| Standard Deviation | 1.41 | 89.8 | 1.41 | 125 | |
| CV (%) | 4.9 | 6.6 | 5.2 | 8.6 | |
| TNF alpha 2nd Gen | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 6.99 | 383 | 0.662 | 36.9 | |
| Standard Deviation | 0.345 | 20.6 | 7.81 | 408 | |
| CV (%) | 4.9 | 5.4 | 8.5 | 9 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| IL-1 beta/IL-1F2 | EDTA Plasma (n=4) | 106 | 97-120 |
| Heparin Plasma (n=4) | 99 | 99-112 | |
| Serum (n=4) | 105 | 96-112 | |
| IL-6 3rd gen | EDTA Plasma (n=4) | 92 | 86-102 |
| Heparin Plasma (n=4) | 87 | 85-90 | |
| Serum (n=4) | 94 | 90-99 | |
| CCM (n=2) | 104 | 103-106 | |
| Saliva (n=4) | 102 | 102-102 | |
| Urine (n=4) | 98 | 93-104 | |
| IL-8/CXCL8 | EDTA Plasma (n=5) | 89 | 75-102 |
| Heparin Plasma (n=5) | 95 | 88-102 | |
| Serum (n=3) | 92 | 88-97 | |
| TNF alpha 2nd Gen | EDTA Plasma (n=4) | 97 | 89-105 |
| Heparin Plasma (n=4) | 89 | 77-96 | |
| Serum (n=4) | 99 | 92-108 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-1 beta/IL-1F2, IL-6 2nd Gen, IL-8/CXCL8, and TNF alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the respective assays.
Specifications
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Simple Plex Assays FAQsReviews for Simple Plex Cytokine Storm Panel
There are currently no reviews for this product. Be the first to review Simple Plex Cytokine Storm Panel and earn rewards!
Have you used Simple Plex Cytokine Storm Panel?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
