Simple Plex Cytokine Screening Panel 2

Catalog #: SPCKE-PS-010769 Datasheet

Simple Plex 32x6 panel containing IL-10 2nd Gen, IL-2, IFN-gamma 3rd Gen, TNF-alpha 2nd Gen, IL-8/CXCL8, and IL-6 3rd Gen with sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum)

Catalog #	Availability	Size / Price	Qty
ST01E-PS-010769

Control Products Available

899077: Simple Plex Control for Human IFN-gamma (3rd Gen)

894977: Simple Plex Control for Human TNF-alpha

894963: Simple Plex Control for Human IL-2

894950: Simple Plex Control for Human IL-8/CXCL8

897129-SP: Simple Plex Control for Human IL-6 3rd gen

897001-SP: Simple Plex Control for Human IL-10 2nd Gen

All Multiplex Cytokine Assay Kits Simple Plex Assays

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Summary Product Datasheets

Simple Plex Cytokine Screening Panel 2 Summary

The Simple Plex Human Multianalyte Assay is for the detection of human IFNg 3rd gen, IL-10 2nd gen, IL-2, IL-6 3rd gen, IL-8, and TNF-a 2nd gen in serum and plasma (EDTA/Heparin).

Sample Type & Volume Required Per Well	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity	IFNg 3rd gen 0.17 pg/mL; IL-10 2nd gen 0.17pg/mL; IL-2 0.54 pg/mL; IL-6 3rd gen 0.11 pg/mL; IL-8 0.19 pg/mL; TNF-a 2nd gen 0.3 pg/mL
Assay Range	IFNg 3rd gen 0.17 - 4,000 pg/mL; IL-10 2nd gen 0.38 - 1,446 pg/mL; IL-2 0.54 - 2,050 pg/mL; IL-6 3rd gen 0.28 - 2,652pg/mL; IL-8 0.19 - 1,804 pg/mL; TNF-a 2nd gen 0.3 - 1,160 pg/mL

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human IFNg 3rd gen, IL-10 2nd gen, IL-2, IL-6 3rd gen, IL-8, TNF-a 2nd gen.
Cross-reactivity	<0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
IFN-gamma 3rd gen	n	16	16	16	16
	Mean (pg/mL)	21.1	1175	23.4	1192
	Standard Deviation	1.48	33.4	2.52	99.4
	CV (%)	7	2.8	10.8	8.3
IL-10 2nd gen	n	16	16	35	37
	Mean (pg/mL)	8.89	462	10.8	477
	Standard Deviation	0.193	16.0	0.940	24.5
	CV (%)	2.2	3.5	8.7	5.1
IL-2	n	16	16	16	16
	Mean (pg/mL)	15	648	13	608
	Standard Deviation	0.8	32.7	1.13	34
	CV (%)	5.3	5.1	8.7	5.6
IL-6 3rd gen	n	16	16	40	40
	Mean (pg/mL)	23.1	876	23.6	1008
	Standard Deviation	0.899	72.1	1.82	77.7
	CV (%)	3.9	8.2	7.7	7.7
IL-8	n	16	16	16	16
	Mean (pg/mL)	28.7	1362	27.3	1463
	Standard Deviation	1.41	89.8	1.41	125
	CV (%)	4.9	6.6	5.2	8.6
TNF alpha 2nd Gen	n	16	16	16	16
	Mean (pg/mL)	6.99	383	0.662	36.9
	Standard Deviation	0.345	20.6	7.81	408
	CV (%)	4.9	5.4	8.5	9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
IFN-gamma	EDTA Plasma (n=4)	89	88-90
	Heparin Plasma (n=4)	90	83-99
	Serum (n=4)	95	83-102
IL-10 2nd gen	EDTA Plasma (n=4)	91	84-93
	Heparin Plasma (n=4)	91	79-95
	CCM (n=2)	101	100-103
	Serum (n=4)	95	93-97
IL-2	EDTA Plasma (n=4)	86	69-100
	Heparin Plasma (n=4)	90	70-103
	Serum (n=4)	92	69-102
IL-6 3rd gen	EDTA Plasma (n=4)	92	86-102
	Heparin Plasma (n=4)	87	85-90
	CCM (n=2)	104	103-106
	Saliva (n=4)	102	102-102
	Urine (n=4)	98	93-104
	Serum (n=4)	94	90-99
IL-8	EDTA Plasma (n=5)	89	75-102
	Heparin Plasma (n=5)	95	88-102
	Serum (n=3)	92	88-97
TNF alpha 2nd Gen	EDTA Plasma (n=4)	97	89-105
	Heparin Plasma (n=4)	89	77-96
	Serum (n=4)	99	92-108