Sheep IgG NorthernLights™ NL557-conjugated Antibody

Catalog #: NL010
24 Citations 4 Reviews Datasheet

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed

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NL010

Detection of Sheep IgG Primary Antibody by Immunocytochemistry.

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Product Details

Citations (24)

FAQs

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Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Sheep IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity

Sheep

Specificity

Sheep IgG (H+L)

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Sheep IgG

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Label

NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Use at a 1:200 dilution

Immunohistochemistry

Use at a 1:200 dilution

Immunocytochemistry

Use at a 1:200 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Sheep IgG Primary Antibody by Immunocytochemistry. Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in KG-1 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

Citations for Sheep IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets
Authors: Susanne N. Wijesinghe, Amel Badoume, Dominika E. Nanus, Archana Sharma‐Oates, Hussein Farah, Michelangelo Certo et al.
Clinical and Translational Medicine
The expression of IGFBP-5 in the reproductive axis and effect on the onset of puberty in female rats
Authors: Zhiqiu Yao, Maosen Lin, Tao Lin, Xinbao Gong, Pin Qin, Hailing Li et al.
Reproductive Biology and Endocrinology
Systematic single-cell pathway analysis to characterize early Tï¿½cell activation
Authors: JA Bibby, D Agarwal, T Freiwald, N Kunz, NS Merle, EE West, P Singh, A Larochelle, F Chinian, S Mukherjee, B Afzali, C Kemper, NR Zhang
Cell Reports, 2022-11-22;41(8):111697.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Reactive morphology of dividing microglia following kainic acid administration
Authors: Tabitha R. F. Green, Sean M. Murphy, Maria P. Moreno-Montano, Etienne Audinat, Rachel K. Rowe
Frontiers in Neuroscience
Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
Authors: W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun
Nature Immunology, 2022-07-06;23(7):1021-1030.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Development and Preliminary Testing of Porcine Blood-Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins
Authors: Andrew Bond, Vito Bruno, Jason Johnson, Sarah George, Raimondo Ascione
International Journal of Molecular Sciences
Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days
Authors: N Won, J Castillo-P, X Tan, J Ford, D Heath, LI Mazilescu, M Selzner, IM Rogers
Cells, 2022-06-02;11(11):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration
Authors: P Flora, MY Li, PM Galbo, M Astorkia, D Zheng, E Ezhkova
PloS Genetics, 2021-12-14;17(12):e1009948.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
Authors: K Karnas, T Str?czek, C Kapusta, M Lekka, J Duli?ska-L, A Karewicz
International Journal of Nanomedicine, 2021-09-24;16(0):6537-6552.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Survival motor neuron deficiency slows myoblast fusion through reduced myomaker and myomixer expression
Authors: Nikki M. McCormack, Eric Villalón, Coralie Viollet, Anthony R. Soltis, Clifton L. Dalgard, Christian L. Lorson et al.
Journal of Cachexia, Sarcopenia and Muscle

Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
Authors: EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
Cell Stem Cell, 2020-09-17;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells
Authors: H Xi, J Langerman, S Sabri, P Chien, CS Young, S Younesi, M Hicks, K Gonzalez, W Fujiwara, J Marzi, S Liebscher, M Spencer, B Van Handel, D Evseenko, K Schenke-La, K Plath, AD Pyle
Cell Stem Cell, 2020-05-11;0(0):.

Effect of GABA-T on Reproductive Function in Female Rats
Authors: Wenyu Si, Hailing Li, Tiezhu Kang, Jing Ye, Zhiqiu Yao, Ya Liu et al.
Animals (Basel)

Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABAA receptors
Authors: JiaJie Teoh, Narayan Subramanian, Maria Elena Pero, Francesca Bartolini, Ariadna Amador, Ayla Kanber et al.
Neurobiology of Disease

Loss of flow responsive Tie1 results in Impaired Aortic valve remodeling
Authors: Xianghu Qu, Kate Violette, M. K. Sewell-Loftin, Jonathan Soslow, LeShana Saint-Jean, Robert B. Hinton et al.
Developmental Biology

Pathogenesis of hypertension in a mouse model for human CLCN2 related hyperaldosteronism
Authors: C Göppner, IJ Orozco, MB Hoegg-Beil, AH Soria, CA Hübner, FL Fernandes-, S Boulkroun, MC Zennaro, TJ Jentsch
Nat Commun, 2019-10-15;10(1):4678.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development
Authors: So EY, Sun C, Wu KQ et al.
J. Cell. Physiol.

IFN-I and IL-22 mediate protective effects of intestinal viral infection
Authors: JA Neil, Y Matsuzawa-, E Kernbauer-, SL Schuster, S Sota, M Venzon, S Dallari, A Galvao Net, A Hine, D Hudesman, P Loke, TJ Nice, K Cadwell
Nat Microbiol, 2019-06-10;0(0):.
Species: Mouse
Sample Types: Organoid
Applications: IHC

Novel fixed Z-dimension (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging
Authors: Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini et al.
Development

Role of Nesfatin-1 in the Reproductive Axis of Male Rat
Sci Rep, 2016-09-07;6(0):32877.
Applications: IHC

Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
Authors: Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M
Stem Cells Int, 2014-09-10;2014(0):468927.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-P

Antitumor effects of chimeric receptor engineered human T cells directed to tumor stroma.
Authors: Kakarla S, Chow K, Mata M, Shaffer D, Song X, Wu M, Liu H, Wang L, Rowley D, Pfizenmaier K, Gottschalk S
Mol Ther, 2013-06-04;21(8):1611-20.
Species: Human
Sample Types: Whole Tissue
Applications: CAR-T (IHC-P)

Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease.
Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
PLoS ONE, 2012-06-07;7(6):e38615.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
Science, 2010-11-05;330(6005):827-30.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC

FAQs

Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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