Recombinant Viral HCMV IL-10 Protein

Catalog #: 117-VL
11 Citations Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
117-VL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
117-VL-025

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Product Details

Citations (11)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Viral HCMV IL-10 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit LPS-induced IL‑1 beta secretion by human peripheral blood lymphocytes (PBL). The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Source

E. coli-derived viral IL-10 protein
Ala26-Lys176

Accession #

AAF63437

N-terminal Sequence
Analysis

Ala26

Structure / Form

Noncovalently-linked homodimer

Predicted Molecular Mass

17.6 kDa

Product Datasheets

117-VL (with carrier)

Product Datasheet

117-VL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

117-VL

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

117-VL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen‑stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

References

Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
Spencer, J.V. et al. (2002) J. Virol. 76:1285.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Related Research Areas

Citations for Recombinant Viral HCMV IL-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Challenging the Conventional Interpretation of HCMV Seronegativity
Authors: S Waters, S Lee, A Irish, P Price
Microorganisms, 2021-11-18;9(11):.
Species: Human
Sample Types: Plasma
Applications: ELISA (Standard)
Human cytomegalovirus interleukin-10 enhances matrigel invasion of MDA-MB-231 breast cancer cells
Authors: CA Valle Oseg, JV Spencer
Cancer Cell Int, 2017-02-13;17(0):24.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
Sci Rep, 2017-01-05;7(0):39710.
Applications: ELISA (Standard)
Human Cytomegalovirus interleukin-10 promotes proliferation and migration of MCF-7 breast cancer cells.
Authors: Bishop R, Valle Oseguera C, Spencer J
Cancer Cell Microenviron, 2015-01-01;2(1):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
PLoS ONE, 2014-02-10;9(2):e88708.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
BMC Biotechnol, 2013-10-05;13(0):82.
Applications: Western Blot
Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
Clin. Cancer Res., 2011-04-13;17(14):4642-9.
Applications: ELISA (Standard)
Human cytomegalovirus suppresses type I interferon secretion by plasmacytoid dendritic cells through its interleukin 10 homolog.
Authors: Chang WL, Barry PA, Szubin R, Wang D, Baumgarth N
Virology, 2009-06-13;390(2):330-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
Eur. J. Immunol., 2008-12-01;38(12):3365-75.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
The downmodulation of the foreign body reaction by cytomegalovirus encoded interleukin-10.
Authors: van Putten SM, Wubben M, Hennink WE, van Luyn MJ, Harmsen MC
Biomaterials, 2008-11-26;30(5):730-5.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo