Recombinant Rat PDGF-AA Protein, CF Summary
Product Specifications
Ser87-Arg196
Analysis
Product Datasheets
1055-AA
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1055-AA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PDGF-AA
The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB,-BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that have five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. Rat PDGF-A chain cDNA encodes a 204 amino acid (aa) residue precursor protein with a 20 aa signal peptide, a 65 aa propeptide that is removed by proteolysis, and a 119 aa mature protein. By alternative splicing, a short form lacking 8 C-terminal aa residues also exists. The long form contains the 8 aa basic insert which promotes intracellular cell retention and association with cell matrix. PDGF-A is expressed in multiple cell types and tissues. Based on PDGF-A knockout studies, PDGF-A appears to be important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles and intestinal villus (1).
- Betshotz, C. et al. (2001) BioEssays 23:494.
- Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
- Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
- LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Citations for Recombinant Rat PDGF-AA Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Temporal control of PDGFRalpha regulates the fibroblast-to-myofibroblast transition in wound healing
Authors: L Yao, BH Rathnakar, HR Kwon, H Sakashita, JH Kim, A Rackley, JJ Tomasek, WL Berry, LE Olson
Cell Reports, 2022-08-16;40(7):111192.
Species: Mouse
Sample Types: Serum
Applications: Bioassay -
Tension Sensor Based on Fluorescence Resonance Energy Transfer Reveals Fiber Diameter-Dependent Mechanical Factors During Myelination
Authors: T Shimizu, H Murakoshi, H Matsumoto, K Ichino, A Hattori, S Ueno, A Ishida, N Tajiri, H Hida
Frontiers in Cellular Neuroscience, 2021-08-02;15(0):685044.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha
Authors: JL Kadrmas, MC Beckerle, M Yoshigi
Sci Rep, 2020-11-09;10(1):19303.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Phthalate inhibits Leydig cell differentiation and promotes adipocyte differentiation
Authors: X Hao, X Guan, X Zhao, M Ji, X Wen, P Chen, F Chen, J Yang, Q Lian, L Ye, H Chen
Chemosphere, 2020-08-06;262(0):127855.
Species: Rat
Sample Types: In Vivo
Applications: Bioassay -
Transplanted Oligodendrocyte Progenitor Cells Survive in the Brain of a Rat Neonatal White Matter Injury Model but Less Mature in Comparison with the Normal Brain
Authors: S Ogawa, M Hagiwara, S Misumi, N Tajiri, T Shimizu, A Ishida, N Suzumori, M Sugiura-Og, H Hida
Cell Transplant, 2020-01-01;29(0):9636897209460.
Species: Rat, Transgenic Rat
Sample Types: Whole Cells
Applications: Bioassay -
Characterization of stem cells associated with seminiferous tubule of adult rat testis for their potential to form Leydig cells
Authors: X Guan, P Chen, X Zhao, X Hao, F Chen, M Ji, X Wen, H Lin, L Ye, H Chen
Stem Cell Res, 2019-10-15;41(0):101593.
Species: Rat
Sample Types: Whole Tissue
Applications: Tissue Culture -
Growth factor signaling regulates mechanical nociception in flies and vertebrates
Authors: R Lopez-Bell, S Puig, PJ Huang, CR Tsai, HN Turner, MJ Galko, HB Gutstein
J. Neurosci., 2019-05-28;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: Bioassay, In Vivo -
Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis
Cell Rep, 2016-11-08;17(7):1832-1844.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Autophagy modulates SNCA/alpha-synuclein release, thereby generating a hostile microenvironment.
Authors: Poehler A, Xiang W, Spitzer P, May V, Meixner H, Rockenstein E, Chutna O, Outeiro T, Winkler J, Masliah E, Klucken J
Autophagy, 2014-01-01;10(12):2171-92.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
BMP4 is required for the initial expression of MITF in melanocyte precursor differentiation from embryonic stem cells.
Authors: Yang J, Wang J, Pan L, Li H, Rao C, Zhang X, Niu G, Qu J, Hou L
Exp Cell Res, 2013-09-27;320(1):54-61.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium.
Authors: Schneider L, Stock CM, Dieterich P, Jensen BH, Pedersen LB, Satir P, Schwab A, Christensen ST, Pedersen SF
J. Cell Biol., 2009-04-06;185(1):163-76.
Species: Hamster, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
Authors: Yang X, Chrisman H, Weijer CJ
Development, 2008-10-02;135(21):3521-30.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay
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Average Rating: 5 (Based on 1 Review)
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Reason for Rating: TRIED IT ON NIH 3T3S MOUSE FIBROBLAST TO ESTABLISH A CHEMOTACTIC GRADIENT AND IT WORKED VERY WELL. THE RESULTS WERE AS EXPECTED.
