Recombinant Rat IL-7 Protein

Catalog #: 7857-RL Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
7857-RL-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7857-RL-010

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Recombinant Rat IL-7 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Source

E. coli-derived rat IL-7 protein
Asp26-Ile154, with an N-terminal Met

Accession #

NP_037242

N-terminal Sequence
Analysis

Starts at Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

15 kDa, reducing conditions

Product Datasheets

7857-RL (with carrier)

Product Datasheet

7857-RL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7857-RL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7857-RL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Rat IL‑7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide. Mature rat IL‑7 shares approximately 88% aa sequence identity with mouse IL‑7 and 58‑60% with human, equine, bovine, ovine, porcine, feline and canine IL‑7. Human and mouse IL‑7 exhibit cross‑species activity (2, 3). IL‑7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL‑7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL‑7 signals through a complex of the IL‑7 Receptor alpha subunit (IL‑7 R alpha, also known as CD127) with the common gamma chain ( gamma _c) (1). The gamma _c is also a subunit of the receptors for IL‑2, ‑4, ‑9, ‑15, and ‑21 (1). IL‑7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive naïve and memory T cells, but undergoes IL‑7‑mediated down‑regulation and shedding during antigen‑driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6‑11). IL‑7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl‑2 (9‑11). It is required for optimal T cell‑dendritic cell interaction (6). IL‑7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL‑7 activation of IL‑7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL‑7 functions in both mouse and human pro‑B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

References