Recombinant Rat IL-4 Protein

Catalog #: 504-RL
13 Citations 2 Reviews Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
504-RL-005/CF
504-RL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
504-RL-005
504-RL-025

R&D Systems Recombinant Proteins and Enzymes

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All IL-4 Proteins and Enzymes

Product Details

Citations (13)

FAQs

Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-4 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using rat splenocytes. Noble, A. and D.M. Kemery (1995) Immunology 85:357. The ED₅₀ for this effect is 0.025-0.25 ng/mL.

Source

E. coli-derived rat IL-4 protein
Cys25-Ser147, with an N-terminal Met

Accession #

P20096.2

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

13.5 kDa

Product Datasheets

504-RL (with carrier)

Product Datasheet

504-RL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

504-RL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

504-RL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 aa signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5 ‑ 7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
McKnight, A.J. et al. (1991) Eur. J. Immunol. 21:1187.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Related Research Areas

Citations for Recombinant Rat IL-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia
Authors: S Kim, Y Son
International Journal of Molecular Sciences, 2021-08-16;22(16):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Rat Cytomegalovirus Virion-Associated Proteins R131 and R129 Are Necessary for Infection of Macrophages and Dendritic Cells
Authors: IKA Jones, NN Haese, P Gatault, ZJ Streblow, TF Andoh, M Denton, CE Streblow, K Bonin, CN Kreklywich, JM Burg, SL Orloff, DN Streblow
Pathogens, 2020-11-19;9(11):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Ethanol Induction of Innate Immune Signals Across BV2 Microglia and SH-SY5Y Neuroblastoma Involves Induction of IL-4 and IL-13
Authors: CJ Lawrimore, LG Coleman, J Zou, FT Crews
Brain Sci, 2019-09-10;9(9):.
Species: Rat
Sample Types: Tissue Explant
Applications: Bioassay
The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
J Neuroinflammation, 2018-08-13;15(1):226.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10)
Authors: S Lively, LC Schlichter
Front Cell Neurosci, 2018-07-24;12(0):215.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
Authors: JE Lim, E Chung, Y Son
Sci Rep, 2017-08-25;7(1):9417.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity
Authors: T Ito, M Ishigami, Y Matsushita, M Hirata, K Matsubara, T Ishikawa, H Hibi, M Ueda, Y Hirooka, H Goto, A Yamamoto
Sci Rep, 2017-03-08;7(0):44043.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke
Authors: HT Lee, SP Liu, CH Lin, SW Lee, CY Hsu, HK Sytwu, CH Hsieh, WC Shyu
Theranostics, 2017-02-08;7(4):855-875.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states.
Authors: Siddiqui T, Lively S, Ferreira R, Wong R, Schlichter L
PLoS ONE, 2014-08-22;9(8):e106087.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Neuronal synapse formation induced by microglia and interleukin 10.
Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
PLoS ONE, 2013-11-22;8(11):e81218.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
Cell. Signal., 2008-10-12;21(1):151-60.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
Authors: Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M
J. Clin. Invest., 2006-03-23;116(4):905-15.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Comparative analysis of the fate of donor dendritic cells and B cells and their influence on alloreactive T cell responses under tacrolimus immunosuppression.
Authors: Azhipa O, Kimizuka K, Nakao A, Toyokawa H, Okuda T, Neto JS, Alber SM, Kaizu T, Thomson AW, Demetris AJ, Murase N
Clin. Immunol., 2005-02-01;114(2):199-209.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay