Recombinant Rat IL-23 Protein Summary
Product Specifications
| Rat IL-23 p40 (Met23 - Ser335) Accession # NP_072133 |
IGSGSSRGGSGSGGSGGGGSK | Rat IL-23 p19 (Leu20 - Ala196) Accession # NP_569094 |
8-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
3136-RL (with carrier)
3136-RL/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3136-RL
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3136-RL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-23
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. The rat p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. The mature rat p19 protein shares 88%, 78%, 76%, 75%, 71%, and 70% aa sequence identity with mouse, human, canine, equine, guinea pig, and bovine, respectively. Activated macrophages and dendritic cells express p19 and p40 concurrently to produce IL-23 (1, 4). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R) (3). IL-23 and IL-12 have overlapping but distinct biological activities. IL-12 drives development of Th1 cells and induces production of IFN-gamma by NK cells; IL-23 induces proliferation of Th17 cells and CD4+ memory T cells distinct from Th1 which produce IL-17, a potent proinflammatory cytokine (2). IL-23 also drives IL-17 production by NK cells and neutrophils (6). While both IL-12 and IL-23 pathways respond to infectious agents, the IL-23 - IL-17 immune pathway induces the earliest recruitment of neutrophils to the site of infection while the more classic host defense and cytotoxic response is stimulated by IL-12 (5). Dysregulation of the IL-23 - IL-17 immune pathway has a key role in organ-specific autoimmune inflammatory tissue destruction (2, 7).
- Oppmann, B. et al. (2000) Immunity 13:715.
- McKenzie, B.S. et al. (2006) Trends Immunol. 27:17.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Cua, D.J. et al. (2003) Nature 421:744.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
- Langrish, C.L. et al. (2004) Immunol. Rev. 202:96.
- Murphy, C.A. et al. (2003) J. Exp. Med. 198:1951.
Citations for Recombinant Rat IL-23 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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TGF? signaling pathway is altered by HLA-B27 expression, resulting in pathogenic consequences relevant for spondyloarthritis
Authors: Lauraine, M;de Taffin de Tilques, M;Melamed-Kadosh, D;Cherqaoui, B;Rincheval, V;Prevost, E;Rincheval-Arnold, A;Cela, E;Admon, A;Guénal, I;Araujo, LM;Breban, M;
Arthritis research & therapy
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Activated pathogenic Th17 lymphocytes induce hypertension following high-fructose intake in Dahl salt-sensitive (SS) but not Dahl salt-resistant (SR) rats
Authors: E Lee, N Kim, J Kang, S Yoon, HA Lee, H Jung, SH Kim, I Kim
Dis Model Mech, 2020-05-27;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Resident Vdelta1+ gammadelta T cells control early infiltration of neutrophils after Escherichia coli infection via IL-17 production.
Authors: Shibata K, Yamada H, Hara H, Kishihara K, Yoshikai Y
J. Immunol., 2007-04-01;178(7):4466-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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