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Recombinant Rat IL-10 Protein

Catalog #: 522-RLB
14 Citations 2 Reviews Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
522-RLB-025/CF

With Carrier

Catalog # Availability Size / Price Qty
522-RLB-025
Recombinant Rat IL-10 Protein Bioactivity
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Product Details
Citations (14)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-10 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.12-0.72 ng/mL.
Source
E. coli-derived rat IL-10 protein
Ser19-Asn178
Accession #
P29456.1
N-terminal Sequence
Analysis
Ser19
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
19 kDa (monomer)
SDS-PAGE
16 kDa, reducing conditions

Product Datasheets

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522-RLB (with carrier)

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522-RLB/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

522-RLB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

522-RLB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Rat IL-10 Protein Bioactivity View Larger

Recombinant Rat IL-10 (Catalog # 522-RLB) promotes MC/9-2 mouse mast cell proliferation. The ED50 for this effect is 0.12-0.72 ng/mL.

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%-79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1.
  9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  10. Syto, R. et al. (1998) Biochemistry 37:16943.
  11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Citations for Recombinant Rat IL-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia
    Authors: S Kim, Y Son
    International Journal of Molecular Sciences, 2021-08-16;22(16):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10)
    Authors: S Lively, LC Schlichter
    Front Cell Neurosci, 2018-07-24;12(0):215.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat
    Authors: T Erkens, N Goeminne, A Kegels, M Byloos, P Vinken
    J Pharmacol Toxicol Methods, 2018-01-31;91(0):43-49.
    Species: Rat
    Sample Types: Serum
    Applications: ELISA (Standard)
  4. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
    Authors: JE Lim, E Chung, Y Son
    Sci Rep, 2017-08-25;7(1):9417.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
    Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
    EMBO Mol Med, 2017-03-01;9(3):337-352.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats
    Front Microbiol, 2016-12-15;7(0):2005.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  7. Interleukin-10 Contributes to Therapeutic Effect of Mesenchymal Stem Cells for Acute Liver Failure via Signal Transducer and Activator of Transcription 3 Signaling Pathway.
    Authors: Hu-Cheng Ma, Xin Wang, Min-Na Wu, Xin Zhao, Xian-Wen Yuan, Xiao-Lei Shi
    Chinese Medical Journal, 2016-04-20;0(0):0366-6999.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states.
    Authors: Siddiqui T, Lively S, Ferreira R, Wong R, Schlichter L
    PLoS ONE, 2014-08-22;9(8):e106087.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. LIMLE, a new molecule over-expressed following activation, is involved in the stimulatory properties of dendritic cells.
    Authors: Le Texier L, Durand J, Lavault A, Hulin P, Collin O, Le Bras Y, Cuturi M, Chiffoleau E
    PLoS ONE, 2014-04-04;9(4):e93894.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2013-07-08;288(33):23776-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
    Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2008-05-16;49(9):3999-4007.
    Species: Rat
    Sample Types: Aqueous Humor
    Applications: Bioassay
  12. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008;26(4):1047-55.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Developmet
  13. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005-10-01;33(10):2317-22.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  14. Lipopolysaccharide-induced increase in signalling in hippocampus is abrogated by IL-10--a role for IL-1 beta?
    Authors: Walsh C, Delaney A, Nolan Y, Campbell VA
    J. Neurochem., 2004-02-01;88(3):635-46.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Rat IL-10 Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Rat IL-10 Protein
By Anonymous on 05/24/2022
Application: Immunoassay Standard
Immunoassay Standard Recombinant Rat IL-10 Protein 522-RLB
Recombinant Rat IL-10 Protein
By Anonymous on 11/20/2021
Application: MI
Reason for Rating: After release in vitro IL-10 was quantified using a multiplex assay. Also used in vivo and had excellent biological activity.