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Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF

Catalog #: 583-IC Datasheet
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583-IC-050
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Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.

When 5 x 104 cells/well are added to rrICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >20% will adhere after 1 hour incubation at 37 °C.

Also supports adhesion (40-70%) of PMA treated rat Nb-211 cells when coated at 12.5 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived rat ICAM-1/CD54 protein
Rat ICAM-1
(Gln28 - Thr493)
Accession # Q00238		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
Q00238
N-terminal Sequence
Analysis
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
78 kDa (monomer)
SDS-PAGE
110-125 kDa, reducing conditions

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583-IC

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

583-IC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

References
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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