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Recombinant Rat GDNF Protein

Catalog #: 512-GF
46 Citations 3 Reviews Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
512-GF-010/CF
512-GF-050/CF

With Carrier

Catalog # Availability Size / Price Qty
512-GF-010
512-GF-050
512-GF-01M
Recombinant Rat GDNF Protein Bioactivity
2 Images
Product Details
Citations (46)
FAQs
Supplemental Products
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat GDNF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha ‑1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR) at 1 µg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein
Ser78-Ile211
Accession #
Q07731
N-terminal Sequence
Analysis
Ser78
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15 kDa (monomer)

Product Datasheets

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512-GF (with carrier)

Product Datasheet

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512-GF/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

512-GF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

512-GF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Rat GDNF Protein Bioactivity View Larger

Recombinant Rat GDNF (Catalog # 512‑GF) promotes the survival of chick embryonic dorsal root ganglia neurons. The ED50 for this effect is 1-3 ng/mL.

SDS-PAGE Recombinant Rat GDNF Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Rat GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19 kDa and 33 kDa, respectively.

Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name
Glial Cell line-derived Growth Factor
Entrez Gene IDs
2668 (Human); 25453 (Rat)
Alternate Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

Citations for Recombinant Rat GDNF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. GDNF facilitates cognitive function recovery following neonatal surgical-induced learning and memory impairment via activation of the RET pathway and modulation of downstream effectors PKM? and Kalirin in rats
    Authors: Chen, Y;Zheng, YX;Li, YZ;Jia, Z;Yuan, Y;
    Brain research bulletin
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo assay
  2. Serum amyloid A3 fuels a feed-forward inflammatory response to the bacterial amyloid curli in the enteric nervous system
    Authors: Verstraelen, P;Van Remoortel, S;De Loose, N;Verboven, R;Garcia-Diaz Barriga, G;Christmann, A;Gries, M;Bessho, S;Li, J;Guerra, C;Tükel, Ç;Ibiza Martinez, S;Schäfer, KH;Timmermans, JP;De Vos, WH;
    Cellular and molecular gastroenterology and hepatology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Utilization of the Rat Tibial Nerve Transection Model to Evaluate Cellular and Molecular Mechanisms Underpinning Denervation-Mediated Muscle Injury
    Authors: Doherty, C;Lodyga, M;Correa, J;Di Ciano-Oliveira, C;Plant, PJ;Bain, JR;Batt, J;
    International journal of molecular sciences
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Architecture and regulation of a GDNF-GFR?1 synaptic adhesion assembly
    Authors: Houghton, FM;Adams, SE;Ríos, AS;Masino, L;Purkiss, AG;Briggs, DC;Ledda, F;McDonald, NQ;
    Nature communications
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A functional network of highly pure enteric neurons in a dish
    Authors: M Caillaud, ME Le Dréan, A De-Guilhem, C Le Berre-S, J Montnach, S Nedellec, G Loussouarn, V Paillé, M Neunlist, H Boudin
    Frontiers in Neuroscience, 2023-01-06;16(0):1062253.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration
    Authors: G D'Este, M Stazi, S Negro, A Megighian, F Lista, O Rossetto, C Montecucco, M Rigoni, M Pirazzini
    International Journal of Molecular Sciences, 2022-01-21;23(3):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Cell-fate transition and determination analysis of mouse male germ cells throughout development
    Authors: J Zhao, P Lu, C Wan, Y Huang, M Cui, X Yang, Y Hu, Y Zheng, J Dong, M Wang, S Zhang, Z Liu, S Bian, X Wang, R Wang, S Ren, D Wang, Z Yao, G Chang, F Tang, XY Zhao
    Nature Communications, 2021-11-25;12(1):6839.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Glial cell-derived neurotrophic factor decrease may mediate learning, memory and behavior impairments in rats after neonatal surgery
    Authors: Y Xie, W Zhao, Z Zuo
    Brain research bulletin, 2021-10-30;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  9. Neuro-mesenchymal units control ILC2 and obesity via a brain-adipose circuit
    Authors: F Cardoso, RGJ Klein Wolt, C Godinho-Si, RG Domingues, H Ribeiro, JA da Silva, I Mahú, AI Domingos, H Veiga-Fern
    Nature, 2021-08-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro
    Authors: S Takaku, M Tsukamoto, N Niimi, H Yako, K Sango
    International Journal of Molecular Sciences, 2021-03-15;22(6):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3
    Authors: CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in M�ller Glial regenerative potential
    Authors: LS VandenBosc, SG Wohl, MS Wilken, M Hooper, C Finkbeiner, K Cox, L Chipman, TA Reh
    Sci Rep, 2020-08-12;10(1):13615.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Short Time Exposure to Low Concentration of Zinc Oxide Nanoparticles Up-regulates Self-renewal and Spermatogenesis-Related Gene Expression
    Authors: A Javadi, S Mokhtari, SF Moraveji, FA Sayahpour, M Farzaneh, H Gourabi, F Esfandiari
    Int. J. Biochem. Cell Biol., 2020-08-06;127(0):105822.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Motoneuron expression profiling identifies an association between an axonal splice variant of HDGF-related protein 3 and peripheral myelination
    Authors: BE Kerman, S Genoud, B Kurt Vatan, AM Denli, SG Ghosh, X Xu, GW Yeo, JB Aimone, FH Gage
    J. Biol. Chem., 2020-07-09;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  15. GCaMP as an indirect measure of electrical activity in rat trigeminal ganglion neurons
    Authors: JE Hartung, MS Gold
    Cell Calcium, 2020-05-30;89(0):102225.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Protocol Optimization for the Production of the Non-Cytotoxic J&deltaNI5 HSV Vector Deficient in Expression of Immediately Early Genes
    Authors: S Kuroda, Y Miyagawa, Y Sato, M Yamamoto, K Adachi, H Kinoh, WF Goins, JB Cohen, JC Glorioso, N Taniai, H Yoshida, T Okada
    Mol Ther Methods Clin Dev, 2020-03-17;17(0):612-621.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Biological and RNA regulatory function of MOV10 in mammalian germ cells
    Authors: K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng
    BMC Biol., 2019-05-14;17(1):39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis
    Authors: C Wu, ME Watts, LL Rubin
    Cell Rep, 2019-01-29;26(5):1143-1156.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells
    Authors: Y Bai, C Zhu, M Feng, H Wei, L Li, X Tian, Z Zhao, S Liu, N Ma, X Zhang, R Shi, C Fu, Z Wu, S Zhang
    Stem Cell Res Ther, 2018-07-18;9(1):200.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Neuronal subtype determines HSV-1 Latency-Associated-Transcript (LAT) promoter activity during latency
    Authors: JR Cabrera, AJ Charron, DA Leib
    J. Virol., 2018-06-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  21. Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS
    Authors: KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo
    Cell Death Dis, 2018-05-22;9(6):596.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. DNA methylation of the Fthl17 5'-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells
    Authors: N Aoki, K Mochizuki, Y Matsui
    PLoS ONE, 2017-02-16;12(2):e0172219.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. In�Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells
    Cell Rep, 2016-12-06;17(10):2789-2804.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. A system to study mechanisms of neuromuscular junction development and maintenance
    Development, 2016-05-25;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  25. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells
    Authors: A Suzuki, M Hirasaki, T Hishida, J Wu, D Okamura, A Ueda, M Nishimoto, Y Nakachi, Y Mizuno, Y Okazaki, Y Matsui, JC Izpisua Be, A Okuda
    Nat Commun, 2016-03-30;7(0):11056.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development.
    Authors: Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G
    PLoS ONE, 2015-06-17;10(6):e0129944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Spermatogonial stem cell enrichment using simple grafting of testis and in vitro cultivation.
    Authors: Lim, Jung Jin, Seol, Dong Won, Choi, Kyung He, Shin, Dong Hyu, Kim, Hyung Jo, Song, Seung-Hu, Lee, Dong Ryu
    Sci Rep, 2014-08-01;4(0):5923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  28. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014-07-27;514(7520):98-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors.
    Authors: Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G
    EMBO J, 2014-06-11;33(14):1582-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
    Authors: Lim J, Shim M, Lee J, Lee D
    PLoS ONE, 2014-04-01;9(4):e90454.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
    Authors: Matsumoto, Shinji, Fujii, Shinsuke, Sato, Akira, Ibuka, Souji, Kagawa, Yoshinor, Ishii, Masaru, Kikuchi, Akira
    EMBO J, 2014-02-20;33(7):702-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases.
    Authors: Sakuraba, Juichi, Shintani, Takafumi, Tani, Sachiko, Noda, Masaharu
    J Biol Chem, 2013-06-28;288(32):23421-31.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons.
    Authors: Hong, Weimin C, Amara, Susan G
    FASEB J, 2013-04-23;27(8):2995-3007.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  34. The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells.
    Authors: Dovere, Lisa, Fera, Stefania, Grasso, Margheri, Lamberti, Dante, Gargioli, Cesare, Muciaccia, Barbara, Lustri, Anna Mar, Stefanini, Mario, Vicini, Elena
    PLoS ONE, 2013-04-22;8(4):e59431.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling.
    Authors: Gui T, Sun Y, Gai Z, Shimokado A, Muragaki Y, Zhou G
    Dev Biol, 2013-03-26;377(2):415-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  36. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013-03-20;33(12):5399-410.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
    Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
    Mol Cell Neurosci, 2012-12-20;54(0):30-43.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  38. Non-viral gene therapy for GDNF production in RCS rat: the crucial role of the plasmid dose.
    Gene Ther., 2011-10-13;19(9):886-98.
    Applications: ELISA (Standard), Western Blot
  39. Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos.
    Authors: Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A
    Development, 2010-03-01;137(5):693-704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Long-term culture of male germline stem cells from hamster testes.
    Authors: Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T
    Biol. Reprod., 2007-12-19;78(4):611-7.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation.
    Authors: He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M
    Stem Cells, 2007-10-25;26(1):266-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor.
    Authors: Garbayo E, Ansorena E, Lanciego JL, Aymerich MS, Blanco-Prieto MJ
    Int J Pharm, 2007-04-06;344(1):9-15.
    Species: Rat
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, Western Blot
  43. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2006-11-10;282(1):721-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
    Authors: Murata T, Tsuboi M, Hikita K, Kaneda N
    J. Biol. Chem., 2006-06-13;281(32):22503-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
    Authors: Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K
    J. Clin. Invest., 2005-08-18;115(9):2393-401.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  46. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
    Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
    Biochemistry, 2005-02-22;44(7):2545-54.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay, ELISA Developmet

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Recombinant Rat GDNF Protein
By Anonymous on 03/11/2020
Application: Tested with ELISA before using in a rat model.
Recombinant Rat GDNF Protein
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