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Recombinant Rat FGF basic/FGF2/bFGF Protein

Catalog #: 3339-FB
35 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
3339-FB-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3339-FB-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (35)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat FGF basic/FGF2/bFGF Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.3-1.8 ng/mL.
Source
E. coli-derived rat FGF basic/FGF2/bFGF protein
Ala11-Ser154
Accession #
P13109
N-terminal Sequence
Analysis
Ala11
Predicted Molecular Mass
16.2 kDa

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3339-FB (with carrier)

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3339-FB/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3339-FB

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3339-FB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-23 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells (5). The 18 kDa rat sequence has 98% amino acid (aa) identity with mouse, and 96-97% aa identity with human, bovine and sheep FGF basic (6, 7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic over-expression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

References
  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
  5. Quarto, N. et al. (2005) Gene 356:49.
  6. Tsuneto, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:1239.
  7. Shimasaki, S. et al. (1988) Biochem. Biophys. Res. Commun. 157:256.
Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Citations for Recombinant Rat FGF basic/FGF2/bFGF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. In vitro cytotoxicity assessment of ruxolitinib on oligodendrocyte precursor cell and neural stem/progenitor cell populations
    Authors: Lim, CW;Hamanaka, G;Liang, AC;Chan, SJ;Ling, KH;Lo, EH;Arai, K;Cheah, PS;
    Neurotoxicology
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular units
    Authors: Wu, Y;Sun, J;Lin, Q;Wang, D;Hai, J;
    Neural regeneration research
    Applications: Bioassay
  3. Neuroprotective Effect of Bcl-2 on Lipopolysaccharide-Induced Neuroinflammation in Cortical Neural Stem Cells
    Authors: SY Park, JS Han
    International Journal of Molecular Sciences, 2022-06-07;23(12):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Photobiomodulation Promotes Repair Following Spinal Cord Injury by Regulating the Transformation of A1/A2 Reactive Astrocytes
    Authors: X Wang, Z Zhang, Z Zhu, Z Liang, X Zuo, C Ju, Z Song, X Li, X Hu, Z Wang
    Frontiers in Neuroscience, 2021-11-02;15(0):768262.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Tension Sensor Based on Fluorescence Resonance Energy Transfer Reveals Fiber Diameter-Dependent Mechanical Factors During Myelination
    Authors: T Shimizu, H Murakoshi, H Matsumoto, K Ichino, A Hattori, S Ueno, A Ishida, N Tajiri, H Hida
    Frontiers in Cellular Neuroscience, 2021-08-02;15(0):685044.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Real-time assessment of guided bone regeneration in critical size mandibular bone defects in rats using collagen membranes with adjunct fibroblast growth factor-2
    Authors: M Furuhata, T Takayama, T Yamamoto, Y Ozawa, M Senoo, M Ozaki, S Yamano, S Sato
    Journal of dental sciences, 2021-04-03;16(4):1170-1181.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Alcohol increases exosome release from microglia to promote complement C1q induced cellular death of proopiomelanocortin neurons in the hypothalamus in a rat model of fetal alcohol spectrum disorders
    Authors: S Mukherjee, MA Cabrera, NI Boyadjieva, G Berger, B Rousseau, DK Sarkar
    J. Neurosci., 2020-09-04;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Long noncoding RNA mediates stroke-induced neurogenesis
    Authors: B Fan, W Pan, X Wang, M Wei, A He, A Zhao, M Chopp, ZG Zhang, XS Liu
    Stem Cells, 2020-05-02;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
    Authors: M Nakamura, H Nishida, K Yoshizaki, H Akiyoshi, S Hatoya, K Sugiura, T Inaba
    J. Vet. Med. Sci., 2020-04-06;0(0):.
    Species: Rat
    Sample Types: cell culture
  10. Reducing Th2 inflammation through neutralizing IL-4 antibody rescues myelination in IUGR rat brain
    Authors: AE Zanno, MA Romer, L Fox, T Golden, L Jaeckle-Sa, RA Simmons, JB Grinspan
    J Neurodev Disord, 2019-12-16;11(1):34.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Overexpression of cortistatin alleviates oxygen/glucose-deprivation-induced ER stress and prompts neural stem cell proliferation via SSTR2
    Authors: X Liang, Q Mao, D Huang, J Tang, J Zheng
    Exp. Mol. Pathol., 2019-12-03;0(0):104351.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Mitochondria damaged by Oxygen Glucose Deprivation can be Restored through Activation of the PI3K/Akt Pathway and Inhibition of Calcium Influx by Amlodipine Camsylate
    Authors: HH Park, MH Han, H Choi, YJ Lee, JM Kim, JH Cheong, JI Ryu, KY Lee, SH Koh
    Sci Rep, 2019-10-31;9(1):15717.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Dopamine neuron induction and the neuroprotective effects of thyroid hormone derivatives
    Authors: EH Lee, SM Kim, CH Kim, SH Pagire, HS Pagire, HY Chung, JH Ahn, CH Park
    Sci Rep, 2019-09-20;9(1):13659.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery
    Authors: SR Sankavaram, R Hakim, R Covacu, A Frostell, S Neumann, M Svensson, L Brundin
    Stem Cell Reports, 2019-04-25;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Implantation of engineered conduction tissue in the rat heart
    Authors: W Zhang, X Li, S Sun, X Zhang
    Mol Med Rep, 2019-02-05;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function
    Authors: X Sun, Y Zhu, HY Yin, ZY Guo, F Xu, B Xiao, WL Jiang, WM Guo, HY Meng, SB Lu, Y Wang, J Peng
    Stem Cell Res Ther, 2018-05-11;9(1):133.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Dephosphorylation is the mechanism of fibroblast growth factor inhibition of guanylyl cyclase-B
    Authors: JW Robinson, JR Egbert, J Davydova, H Schmidt, LA Jaffe, LR Potter
    Cell. Signal., 2017-09-28;40(0):222-229.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Oncogenic Characterization and Pharmacologic Sensitivity of Activating Fibroblast Growth Factor Receptor (FGFR) Genetic Alterations to the Selective FGFR Inhibitor Erdafitinib
    Authors: JD Karkera, G Martinez C, K Bell, D Gaffney, JC Portale, A Santiago-W, C Moy, P King, M Sharp, R Bahleda, FR Luo, JD Alvarez, MV Lorenzi, SJ Platero
    Mol. Cancer Ther., 2017-04-17;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Sox10(+) adult stem cells contribute to biomaterial encapsulation and microvascularization
    Authors: D Wang, A Wang, F Wu, X Qiu, Y Li, J Chu, WC Huang, K Xu, X Gong, S Li
    Sci Rep, 2017-01-10;7(0):40295.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  20. In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue
    Authors: M Vitacolonn
    Cell Tissue Bank, 2016-12-21;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Hippocalcin Is Required for Astrocytic Differentiation through Activation of Stat3 in Hippocampal Neural Precursor Cells
    Authors: Joong-Soo Han
    Front Mol Neurosci, 2016-10-28;9(0):110.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats
    Sci Rep, 2016-09-19;6(0):33428.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  23. Vitamin C facilitates dopamine neuron differentiation in fetal midbrain through TET1- and JMJD3-dependent epigenetic control manner.
    Authors: He X, Kim M, Kim S, Yi S, Rhee Y, Kim T, Lee E, Park C, Dixit S, Harrison F, Lee S
    Stem Cells, 2015-04-01;33(0):1320.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.
    Authors: Xu W, Sun Y, Zhang J, Xu K, Pan L, He L, Song Y, Njunge L, Xu Z, Chiang M, Sung K, Chuong C, Yang L
    Stem Cells Dev, 2015-01-19;24(7):857-68.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Change of fate commitment in adult neural progenitor cells subjected to chronic inflammation.
    Authors: Covacu R, Perez Estrada C, Arvidsson L, Svensson M, Brundin L
    J Neurosci, 2014-08-27;34(35):11571-82.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats.
    Authors: Romariz S, Paiva D, Valente M, Barnabe G, Frussa-Filho R, Barbosa-Silva R, Calcagnotto M, Longo B
    BMC Neurosci, 2014-08-02;15(0):94.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  27. TGF-beta signalling in the adult neurogenic niche promotes stem cell quiescence as well as generation of new neurons.
    Authors: Kandasamy M, Lehner B, Kraus S, Sander P, Marschallinger J, Rivera F, Trumbach D, Ueberham U, Reitsamer H, Strauss O, Bogdahn U, Couillard-Despres S, Aigner L
    J Cell Mol Med, 2014-04-30;18(7):1444-59.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Pathogen sensing pathways in human embryonic stem cell derived-endothelial cells: role of NOD1 receptors.
    Authors: Reed, Daniel M, Foldes, Gabor, Gatheral, Timothy, Paschalaki, Koralia, Lendvai, Zsuzsann, Bagyura, Zsolt, Nemeth, Tamas, Skopal, Judit, Merkely, Bela, Telcian, Aurica G, Gogsadze, Leila, Edwards, Michael, Gough, Peter J, Bertin, John, Johnston, Sebastia, Harding, Sian E, Mitchell, Jane A
    PLoS ONE, 2014-04-01;9(4):e91119.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  29. Meclozine facilitates proliferation and differentiation of chondrocytes by attenuating abnormally activated FGFR3 signaling in achondroplasia.
    Authors: Matsushita, Masaki, Kitoh, Hiroshi, Ohkawara, Bisei, Mishima, Kenichi, Kaneko, Hiroshi, Ito, Mikako, Masuda, Akio, Ishiguro, Naoki, Ohno, Kinji
    PLoS ONE, 2013-12-04;8(12):e81569.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
    Authors: Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J
    Dev Biol, 2013-11-08;385(2):291-303.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  31. The effects of topographical patterns and sizes on neural stem cell behavior.
    Authors: Qi, Lin, Li, Ning, Huang, Rong, Song, Qin, Wang, Long, Zhang, Qi, Su, Ruigong, Kong, Tao, Tang, Minglian, Cheng, Guosheng
    PLoS ONE, 2013-03-18;8(3):e59022.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Wnt-frizzled signaling is part of an FGF-induced cascade that promotes lens fiber differentiation.
    Authors: Dawes L, Sugiyama Y, Tanedo A, Lovicu F, McAvoy J
    Invest Ophthalmol Vis Sci, 2013-03-01;54(3):1582-90.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  33. Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
    Authors: Asumda FZ, Chase PB
    BMC Cell Biol., 2011-10-12;12(0):44.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
    Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
    Cell. Signal., 2008-10-12;21(1):151-60.
    Species: Mouse, Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay
  35. A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
    Authors: Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
    Stem Cells, 2007-09-27;26(1):163-72.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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  1. What receptors does FGF basic bind?
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