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Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

Catalog #: 2159-FA
3 Citations Datasheet
Catalog # Availability Size / Price Qty
2159-FA-050
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Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED50 for this effect is 0.05-0.25 µg/mL in the presence of 100 ng/mL recombinant rat Fas Ligand.
Source
Mouse myeloma cell line, NS0-derived rat Fas/TNFRSF6/CD95 protein
Rat Fas
(Gln22 - Lys170)
Accession # NP_631933		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
NP_631933
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.3 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions

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2159-FA

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2159-FA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1 ‑ 3). Rat Fas cDNA encodes 324 amino acids (aa) that include a 21 aa signal peptide, a 150 aa extracellular domain (ECD) that contains three cysteine-rich TNFR repeats, a 17 aa transmembrane sequence, and a 136 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature rat Fas ECD shares 68% aa sequence identity with mouse Fas, and 56 ‑ 58% with human, feline, bovine and porcine Fas. A 114 aa potential rat Fas isoform contains an alternate start site at aa 211, thus lacking all three TNFR repeats (5). The Fas ligand (FasL, TNFSF6) is a type II transmembrane protein of the TNF family that can be expressed on activated T-lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (6). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation-induced death of T lymphocytes that terminates the immune reaction (1, 2, 7). In immune-privileged tissues, infiltrating Fas-bearing lymphocytes and inflammatory cells are killed by FasL engagement (8). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1 ‑ 3). The Fas pathway also appears to cross-communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

References
  1. Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
  2. Strasser, A. et al. (2009) Immunity 30:180.
  3. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  4. SwissProt accession Q63199.
  5. GenBank protein accession EDM13159.
  6. Thorburn, A. (2003) Cellular Signaling 16:139.
  7. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
  8. Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.
Long Name
Fibroblast-associated
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Citations for Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Apolipoprotein E is a pancreatic extracellular factor that maintains mature ?-cell gene expression
    Authors: AI Mahmoud, FX Galdos, KA Dinan, MP Jedrychows, JC Davis, A Vujic, I Rachmin, C Shigley, JR Pancoast, S Lee, J Hollister-, CM MacGillivr, SP Gygi, DA Melton, GC Weir, RT Lee
    PLoS ONE, 2018-10-10;13(10):e0204595.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. c-Jun N-terminal kinases mediate Fas-induced neurite regeneration in PC12 cells.
    Authors: Waetzig V, Loose K, Haeusgen W, Herdegen T
    Biochem. Pharmacol., 2008-07-19;76(11):1476-84.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Murine coronavirus-induced oligodendrocyte apoptosis is mediated through the activation of the Fas signaling pathway.
    Authors: Liu Y, Zhang X
    Virology, 2006-12-06;360(2):364-75.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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