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Recombinant Rat CX3CL1/Fractalkine Protein

Catalog #: 536-FR
3 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
536-FR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
536-FR-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CX3CL1/Fractalkine Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 0.02-0.08 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat CX3CL1/Fractalkine protein
Rat CX3CL1/Fractalkine
(Gln25-Thr334)
Accession # O55145		 IEGR GGGSGGGSGGGS  HHHHHHHHHH
N-terminus C-terminus
Accession #
O55145
N-terminal Sequence
Analysis
No results obtained: Gln25 predicted
Predicted Molecular Mass
36 kDa
SDS-PAGE
100 kDa, reducing conditions

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536-FR (with carrier)

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536-FR/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

536-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

536-FR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 74 aa or 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino acid sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity.

References
  1. Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
  2. Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
  3. Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
  4. Rossi, D.L. et al. (1998) Genomics 47:163.
Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Rat CX3CL1/Fractalkine Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Fractalkine signaling in microglia contributes to ectopic orofacial pain following trapezius muscle inflammation.
    Authors: Kiyomoto M, Shinoda M, Okada-Ogawa A, Noma N, Shibuta K, Tsuboi Y, Sessle B, Imamura Y, Iwata K
    J Neurosci, 2013-05-01;33(18):7667-80.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons.
    Authors: Cook A, Hippensteel R, Shimizu S, Nicolai J, Fatatis A, Meucci O
    J. Biol. Chem., 2010-02-02;285(14):10563-71.
    Applications: Western Blot
  3. Fractalkine and minocycline alter neuronal activity in the spinal cord dorsal horn.
    Authors: Owolabi SA, Saab CY
    FEBS Lett., 2006-07-07;580(18):4306-10.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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