Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

Catalog #: 10034-EV Datasheet

Catalog #	Availability	Size / Price	Qty
10034-EV-050

Recombinant R. sanguineus Evasin-3 His-tag Protein Bioactivity

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All Evasin-3 Proteins and Enzymes

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Recombinant R. sanguineus Evasin-3 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit CXCL8-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CXCR2. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived r. sanguineus Evasin-3 protein
Leu21-Arg86. with a C-terminal 6-His tag

Accession #

P0C8E8

N-terminal Sequence
Analysis

Leu21

Predicted Molecular Mass

7.8 kDa

SDS-PAGE

8-17 kDa, reducing conditions

Product Datasheets

10034-EV

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10034-EV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant R. sanguineus Evasin-3 (Catalog # 10034-EV) inhibits IL-8/CXCL8-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCXR2. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

Background: Evasin-3

Evasin-3 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-3 encodes an 86 amino acid (aa) precursor, which includes a 20 aa signal peptide and a 66 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown to contain chemokine neutralization activity. These proteins have been identified as chemokine binding proteins (CHPBs) that were termed as Evasins (1,2.3). Evasin-3 belongs to a new class of chemokine binding proteins in that it shows high affinity binding to a very limited set of chemokines, including CXCL8 and CXCL1. Since it is very small molecule Evasin-3 may be therapeutically useful as novel anti-inflammatory agent.

References