Recombinant Mouse VEGF 188 Protein

Catalog #: 7916-MV
1 Citation Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
7916-MV-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7916-MV-010

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Citations (1)

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Recombinant Mouse VEGF 188 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 7-35 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse VEGF protein
Ala27-Arg214

Accession #

Q00731

N-terminal Sequence
Analysis

Ala27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

22.1 kDa (monomer)

SDS-PAGE

28-30 kDa, reducing conditions

Product Datasheets

7916-MV (with carrier)

Product Datasheet

7916-MV/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7916-MV

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7916-MV/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF‑A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1‑3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternate splicing produces isoforms including 121, 145, 165, 183, 189, and 206 amino acid (aa) forms in humans, with 120, 164 and 188 aa isoforms found in mouse (1‑4). Mouse VEGF₁₈₈ shares 98% aa sequence identity with the appropriate isoform in rat, and 89% with human, bovine and canine VEGF. While isoforms VEGF₁₂₀ and VEGF₁₂₁ are freely diffusible, VEGF₁₈₈, VEGF₁₈₉ and VEGF₂₀₆ contain the highest number of basic aa, which bind heparin and tether these isoforms to the cell surface and extracellular matrix (3‑5). Expression of VEGF_188/189 is particularly high in the embryonic lung, where it is produced by type II alveolar epithelia (6). It is thought to be involved in lung, heart and liver vasculogenesis (5, 6). It is not sufficient for vasculogenesis during bone development, but may play a role in bone repair (5, 7). Tumor cell production of VEGF_188/189 correlates with poor prognosis (5). VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt‑1) and VEGF R2 (Flk‑1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF₁₈₈ binds VEGF R2 best when it is cleaved by uPA or plasmin into a 110‑111 aa form (4, 5). Human VEGF₁₆₅ and VEGF₁₈₉ also bind the semaphorin receptor Neuropilin‑1 (8, 9).

References

Breier, G. et al. (1992) Development 114:521.
Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877.
Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
Woolard, J. et al. (2009) Microcirculation 16:572.
Ng, Y.S. et al. (2001) Dev. Dyn. 220:112.
Maes, C. et al. (2004) J. Clin. Endocrinol. 113:188.
Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
Vintonenko, N. et al. (2011) Cell Adh. Migr. 5:332.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Related Research Areas

Citation for Recombinant Mouse VEGF 188 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1