Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Met | Mouse VCAM-1 (Phe25-Glu698) Accession # CAA47989 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
N-terminus | C-terminus | |||
Analysis
Product Datasheets
643-VM
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
643-VM
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 1 mg/mL in sterile, deionized water. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Citations for Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Neutrophil-specific expression of JAK2-V617F or CALRmut induces distinct inflammatory profiles in myeloproliferative neoplasia
Authors: Haage, TR;Charakopoulos, E;Bhuria, V;Baldauf, CK;Korthals, M;Handschuh, J;Müller, P;Li, J;Harit, K;Nishanth, G;Frey, S;Böttcher, M;Fischer, KD;Dudeck, J;Dudeck, A;Lipka, DB;Schraven, B;Green, AR;Müller, AJ;Mougiakakos, D;Fischer, T;
Journal of hematology & oncology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Identification of Tensin-3 as a MALT1 substrate that controls B cell adhesion and lymphoma dissemination
Authors: Juilland, M;Alouche, N;Ubezzi, I;Gonzalez, M;Rashid, HO;Scarpellino, L;Erdmann, T;Grau, M;Lenz, G;Luther, SA;Thome, M;
Proceedings of the National Academy of Sciences of the United States of America
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion
Authors: Park, JS;Kim, JH;Soh, WC;Kim, NY;Lee, KS;Kim, CH;Chung, IJ;Lee, S;Kim, HR;Jun, CD;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
Authors: YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra
Science Advances, 2022-08-24;8(34):eabn5522.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice
Authors: S Sevilla-Mo, P Fuentes, Y Rodríguez-, N Arellano-S, PW Krenn, SI de Val, S Montero-He, J García-Cec, V Burdiel-He, SR Gardeta, N Aguilera-M, C Barrio-Alo, G Crainiciuc, D Bouvard, A García-Par, AG Zapata, A Hidalgo, R Fässler, YR Carrasco, ML Toribio, J Teixidó
European Journal of Immunology, 2022-05-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Thrombin-Activated Platelets Protect Vascular Endothelium against Tumor Cell Extravasation by Targeting Endothelial VCAM-1
Authors: CM Lee, ML Chang, RH Chen, FW Chen, JC Liu, SL Kuo, HH Peng
International Journal of Molecular Sciences, 2022-03-22;23(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Plasma cell dynamics in the bone marrow niche
Authors: Z Benet, Z Jing, DR Fooksman
Cell Reports, 2021-02-09;34(6):108733.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
LFA-1 signals to promote actin polymerization and upstream migration in T cells
Authors: NH Roy, SHJ Kim, A Buffone, D Blumenthal, B Huang, S Agarwal, PL Schwartzbe, DA Hammer, JK Burkhardt
J. Cell. Sci., 2020-09-09;133(17):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo
Authors: V Azcutia, M Kelm, AC Luissint, K Boerner, S Flemming, M Quiros, G Newton, A Nusrat, FW Luscinskas, CA Parkos
Mucosal Immunol, 2020-06-19;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance
Authors: V Barbier, J Erbani, C Fiveash, JM Davies, J Tay, MR Tallack, J Lowe, JL Magnani, DR Pattabiram, AC Perkins, J Lisle, JEJ Rasko, JP Levesque, IG Winkler
Nat Commun, 2020-04-27;11(1):2042.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
beta1-integrin- and KV1.3 channel-dependent signaling stimulates glutamate release from Th17 cells
Authors: K Birkner, B Wasser, T Ruck, C Thalman, D Luchtman, K Pape, S Schmaul, L Bitar, EM Krämer-Alb, A Stroh, SG Meuth, F Zipp, S Bittner
J. Clin. Invest., 2020-02-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
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Integrin CD11b activation drives anti-tumor innate immunity
Authors: MC Schmid, SQ Khan, MM Kaneda, P Pathria, R Shepard, TL Louis, S Anand, G Woo, C Leem, MH Faridi, T Geraghty, A Rajagopala, S Gupta, M Ahmed, RI Vazquez-Pa, DA Cheresh, V Gupta, JA Varner
Nat Commun, 2018-12-19;9(1):5379.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells
Authors: J Chen, N Li, Y Yin, N Zheng, M Min, B Lin, L Zhang, X Long, Y Zhang, Z Cai, S Zhai, J Qin, X Wang
Cell Rep, 2018-12-18;25(12):3393-3404.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and ?1-integrin function
Authors: V Andreani, S Ramamoorth, A Pandey, E Lupar, SL Nutt, T Lämmermann, R Grosschedl
Proc. Natl. Acad. Sci. U.S.A., 2018-09-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus
Authors: FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser
Elife, 2018-09-06;7(0):.
Species: Mouse
Sample Types: Whole Cells
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Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis
Authors: A Farinazzo, S Angiari, E Turano, E Bistaffa, S Dusi, S Ruggieri, R Bonafede, R Mariotti, G Constantin, B Bonetti
Sci Rep, 2018-05-10;8(1):7473.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay -
RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice
Authors: K Morita, C Tokushige, S Maeda, H Kiyose, M Noura, A Iwai, M Yamada, G Kashiwazak, J Taniguchi, T Bando, M Hirata, TR Kataoka, T Nakahata, S Adachi, H Sugiyama, Y Kamikubo
Blood Adv, 2018-03-13;2(5):509-515.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Plasminogen Activator Inhibitor-1 Promotes Neutrophil Infiltration and Tissue Injury on Ischemia-Reperfusion
Authors: M Praetner, G Zuchtriege, M Holzer, B Uhl, J Schaubäche, L Mittmann, M Fabritius, R Fürst, S Zahler, D Funken, M Lerchenber, A Khandoga, S Kanse, K Lauber, F Krombach, CA Reichel
Arterioscler. Thromb. Vasc. Biol., 2018-01-25;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Kindlin-1 promotes pulmonary breast cancer metastasis
Authors: S Sarvi, H Patel, J Li, GL Dodd, H Creedon, M Muir, J Ward, JC Dawson, M Lee, J Culley, DM Salter, AH Sims, A Byron, VG Brunton
Cancer Res., 2018-01-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia
Authors: S Paetau, T Rolova, L Ning, CG Gahmberg
Front Mol Neurosci, 2017-12-22;10(0):431.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex
Authors: CP Basole, RK Nguyen, K Lamothe, A Vang, R Clark, GS Baillie, PM Epstein, S Brocke
Cell. Signal., 2017-08-26;40(0):62-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate
Authors: PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley
J. Leukoc. Biol., 2017-06-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
Nat. Immunol., 2017-04-17;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
Authors: S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra
Nat. Methods, 2017-04-10;14(5):531-538.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration
Authors: ML Estin, SB Thompson, B Traxinger, MH Fisher, RS Friedman, J Jacobelli
Proc. Natl. Acad. Sci. U.S.A, 2017-03-20;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
MFG-E8-derived peptide attenuates adhesion and migration of immune cells to endothelial cells
Authors: Y Hirano, WL Yang, M Aziz, F Zhang, B Sherry, P Wang
J. Leukoc. Biol, 2017-01-17;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
Sci Rep, 2016-11-29;6(0):37914.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Aged neutrophils contribute to the first line of defense in the acute inflammatory response
Blood, 2016-09-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A Critical Role for P2X7 Receptor-Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury
J Immunol, 2016-08-24;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Post-arrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro
Authors: Henriette Rudolph
Eur J Immunol, 2016-07-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Platelets Guide Leukocytes to Their Sites of Extravasation
PLoS Biol, 2016-05-06;14(5):e1002459.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin.
Authors: Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R
Eur J Immunol, 2015-10-06;45(12):3302-12.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Arsenic Exposure Increases Monocyte Adhesion to the Vascular Endothelium, a Pro-Atherogenic Mechanism.
Authors: Lemaire M, Negro Silva L, Lemarie C, Bolt A, Flores Molina M, Krohn R, Smits J, Lehoux S, Mann K
PLoS ONE, 2015-09-02;10(9):e0136592.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
B Lymphocyte-Specific Loss of Ric-8A Results in a Galpha Protein Deficit and Severe Humoral Immunodeficiency.
Authors: Boularan C, Hwang I, Kamenyeva O, Park C, Harrison K, Huang Z, Kehrl J
J Immunol, 2015-07-31;195(5):2090-102.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration.
Authors: Totsuka N, Kim Y, Kanemaru K, Niizuma K, Umemoto E, Nagai K, Tahara-Hanaoka S, Nakahasi-Oda C, Honda S, Miyasaka M, Shibuya K, Shibuya A
Nat Commun, 2014-08-19;5(0):4710.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cytoskeletal regulatory gene expression and migratory properties of B-cell progenitors are affected by the ETV6-RUNX1 rearrangement.
Authors: Palmi C, Fazio G, Savino A, Procter J, Howell L, Cazzaniga V, Vieri M, Longinotti G, Brunati I, Andre V, Della Mina P, Villa A, Greaves M, Biondi A, D'Amico G, Ford A, Cazzaniga G
Mol Cancer Res, 2014-07-24;12(12):1796-806.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Phospholipase D1 mediates lymphocyte adhesion and migration in experimental autoimmune encephalomyelitis.
Authors: Gobel K, Schuhmann M, Pankratz S, Stegner D, Herrmann A, Braun A, Breuer J, Bittner S, Ruck T, Wiendl H, Kleinschnitz C, Nieswandt B, Meuth S
Eur J Immunol, 2014-06-05;44(8):2295-305.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
Authors: Wang X, Rodda L, Bannard O, Cyster J
J Immunol, 2014-04-16;192(10):4601-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Endothelial cell-derived chemerin promotes dendritic cell transmigration.
Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
J Immunol, 2014-01-27;192(5):2366-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Characterization of VCAM-1-binding peptide-functionalized quantum dots for molecular imaging of inflamed endothelium.
Authors: Chen Y, Molnar M, Li L, Friberg P, Gan L, Brismar H, Fu Y
PLoS ONE, 2013-12-31;8(12):e83805.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression.
Authors: Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T
J Immunol, 2013-12-04;192(1):407-17.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
Authors: Lee J, Sohn J, Ryu S, Hong C, Moon K, Park J
J Cell Mol Med, 2013-07-16;17(10):1271-81.
Species: Human
Sample Types: Protein
Applications: Bioassay -
PI3-kinase gamma promotes Rap1a-mediated activation of myeloid cell integrin alpha4beta1, leading to tumor inflammation and growth.
Authors: Schmid M, Franco I, Kang S, Hirsch E, Quilliam L, Varner J
PLoS ONE, 2013-04-02;8(4):e60226.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Toxoplasma gondii modulates the dynamics of human monocyte adhesion to vascular endothelium under fluidic shear stress.
Authors: Harker K, Ueno N, Wang T, Bonhomme C, Liu W, Lodoen M
J Leukoc Biol, 2013-03-13;93(5):789-800.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
J. Immunol., 2012-01-04;188(3):1421-30.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells.
Authors: Moser M, Bauer M, Schmid S, Ruppert R, Schmidt S, Sixt M, Wang HV, Sperandio M, Fassler R
Nat. Med., 2009-02-22;15(3):300-5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit.
Authors: Sasaki K, Pardee AD, Okada H, Storkus WJ
Eur. J. Immunol., 2008-10-01;38(10):2865-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
High pressure promotes monocyte adhesion to the vascular wall.
Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circ. Res., 2007-03-29;100(8):1226-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
J. Leukoc. Biol., 2006-12-18;81(3):748-56.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
Authors: Cardones AR, Murakami T, Hwang ST
Cancer Res., 2003-10-15;63(20):6751-7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Flow Cytometry
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