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Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

Catalog #: 10480-DT Datasheet
Catalog # Availability Size / Price Qty
10480-DT-100
Recombinant Mouse Tyro3/Dtk His-tag Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Tyro3/Dtk His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Tyro3/Dtk His-tag (Catalog # 10480-DT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Catalog # 8310-GS) binds with an ED50 of 0.4-2.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Tyro3/Dtk protein
Ala31-Thr418, with a C-terminal 6-His tag
Accession #
P55144.2
N-terminal Sequence
Analysis
Ala31
Predicted Molecular Mass
42 kDa
SDS-PAGE
60-75 kDa, under reducing conditions

Product Datasheets

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10480-DT

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10480-DT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse Tyro3/Dtk His-tag Protein Binding Activity View Larger

When Recombinant Mouse Tyro3/Dtk His-tag Protein is immobilized at 1 μg/mL (100 μL/well), Recombinant Mouse GAS6 (Full Length) Protein (Catalog # 8310-GS) binds with an ED50 of 0.4-2.4 μg/mL.

SDS-PAGE Recombinant Mouse Tyro3/Dtk His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Tyro3/Dtk His-tag Protein (Catalog # 10480-DT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa.

Background: Tyro3/Dtk

Dtk (Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark), and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. Mouse DTK is a 42kDa protein with a 30 amino acid (aa) signal sequence, 389 aa extracellular domain (ECD), and a 20 aa transmembrane segment. The ECD of mouse DTK shares 85% and 96% sequence identity with the ECD of human and rat respectively. The extracellular domain of these proteins contains two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain. In embryoid stem cells growing in the presence of leukemia inhibitory factor, DTK is abundantly expressed and this level of expression is maintained in differentiating embryoid stem cells and cystic embryoid bodies. In mid-gestational embryos, DTK RNA is expressed in many tissues including brain, eye, thymus, lung, heart, gut, liver, testis and limbs. In contrast, expression of DTK in adult mice becomes restricted to brain, portions of gastrointestinal tract, bladder, testis and ovary.  Overexpression of DTK and several other receptor tyrosine kinase have been found in small cell lung cancer (4).

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  3. Crosier, P. et al. (1994) Growth Factors 11:125.
  4. Hamilton, G. et al. (2015) Oncoscience 2:629.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs
7301 (Human); 22174 (Mouse)
Alternate Names
Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

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