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Recombinant Mouse TIM-3 Fc Chimera Protein, CF

Catalog #: 1529-TM
2 Citations Datasheet
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1529-TM-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TIM-3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin‑9 (Catalog # 2045-GA) at 500 ng/mL can bind recombinant mouse TIM-3 Fc Chimera with an apparent KD <10 nM.
Source
Mouse myeloma cell line, NS0-derived mouse TIM-3 protein
Mouse TIM-3
(Leu22-Arg191)
Accession # AAL65156		 PIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
AAL65156
N-terminal Sequence
Analysis
Leu22 & Asp24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45.6 kDa (monomer)
SDS-PAGE
67-80 kDa, reducing conditions

Product Datasheets

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1529-TM

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1529-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature mouse TIM-3 consists of a 174 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic tail (3). Within the ECD, mouse TIM-3 shares 58% and 75% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (4‑11). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (12). TIM-3 ligation attenuates CD8+ and Th1 cell responses (12-14) and promotes the activity of Treg and myeloid derived suppressor cells (9, 12, 14, 15). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (5). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (11).

References
  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Monney, L. et al. (2002) Nature 415:536.
  9. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  10. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  11. Gleason, M.K. et al. (2012) Blood 119:3064.
  12. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  13. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  14. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  15. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

Citations for Recombinant Mouse TIM-3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity.
    Authors: Matsumura, Takuhiro, Sugawara, Yo, Yutani, Masahiro, Amatsu, Sho, Yagita, Hideo, Kohda, Tomoko, Fukuoka, Shin-Ich, Nakamura, Yutaka, Fukuda, Shinji, Hase, Koji, Ohno, Hiroshi, Fujinaga, Yukako
    Nat Commun, 2015-02-17;6(0):6255.
    Species: Mouse
    Sample Types: Protein
    Applications: Enzyme Assay
  2. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013-03-21;9(3):e1003253.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Bioassay

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