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Recombinant Mouse Tie-2 Fc Chimera Protein, CF

Catalog #: 762-T2
6 Citations Datasheet
Catalog # Availability Size / Price Qty
762-T2-100
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Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Tie-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhAngiopoietin-2 with a linear range of 2‑50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Tie-2 protein
Mouse Tie-2
(Ala23 - Lys744)
Accession # CAA47857		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
CAA47857
N-terminal Sequence
Analysis
Ala23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
107.2 kDa (monomer)
SDS-PAGE
125-135 kDa, reducing conditions

Product Datasheets

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762-T2

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

762-T2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.

References
  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs
7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1

Citations for Recombinant Mouse Tie-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk
    Authors: I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al
    Nature Neuroscience, 2021-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study
    Authors: C Helaine, AE Ferré, MM Leblond, EA Pérès, M Bernaudin, S Valable, E Petit
    Cancers, 2020-11-30;12(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Pericyte-Derived Dickkopf2 Regenerates Damaged Penile Neurovasculature Through an Angiopoietin-1-Tie2 Pathway
    Authors: GN Yin, HR Jin, MJ Choi, A Limanjaya, K Ghatak, NN Minh, J Ock, MH Kwon, KM Song, HJ Park, HM Kim, YG Kwon, JK Ryu, JK Suh
    Diabetes, 2018-03-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Vasculotide reduces pulmonary hyperpermeability in experimental pneumococcal pneumonia
    Authors: B Gutbier, X Jiang, K Dietert, C Ehrler, J Lienau, P Van Slyke, H Kim, VC Hoang, JT Maynes, DJ Dumont, AD Gruber, N Weissmann, TJ Mitchell, N Suttorp, M Witzenrath
    Crit Care, 2017-11-13;21(1):274.
    Applications: Bioassay
  5. Inhibition of Ninjurin 1 restores erectile function through dual angiogenic and neurotrophic effects in the diabetic mouse.
    Authors: Yin, Guo Nan, Choi, Min Ji, Kim, Woo Jean, Kwon, Mi-Hye, Song, Kang-Moo, Park, Jin-Mi, Das, Nando Du, Kwon, Ki-Dong, Batbold, Dulguun, Oh, Goo Taeg, Koh, Gou Youn, Kim, Kyu-Won, Ryu, Ji-Kan, Suh, Jun-Kyu
    Proc Natl Acad Sci U S A, 2014-06-16;111(26):E2731-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody.
    Authors: Popkov M, Jendreyko N, McGavern DB, Rader C, Barbas CF
    Cancer Res., 2005-02-01;65(3):972-81.
    Applications: Binding Assay

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