Recombinant Mouse Thrombospondin-1 Protein, CF

Catalog #: 7859-TH
12 Citations 1 Review Datasheet

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7859-TH-050

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Citations (12)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Thrombospondin-1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.1-0.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Thrombospondin-1 protein

Mouse Thrombospondin-1 (Asn19-Ser1171) Accession # NP_035710	GGGSGGGSGGGS	6-His tag
N-terminus		C-terminus

Accession #

NP_035710

N-terminal Sequence
Analysis

Starts at Asn19

Predicted Molecular Mass

129 kDa

SDS-PAGE

130-155 kDa, reducing conditions

Product Datasheets

7859-TH

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7859-TH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-1

Thrombospondin‑1 (TSP‑1) is a 150‑180 kDa calcium‑sensitive protein that is secreted as a disulfide‑linked homotrimer. Within the Thrombospondin family, TSP‑2 is also homotrimeric, while TSP-3, -4, and -5/COMP are pentameric. TSP‑1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N‑terminal Laminin G‑like globular domain, an extended central region with one vWF‑C domain, 3 TSP type 1 repeats, 3 EGF‑like domains, and 8 TSP type‑3 repeats, and a globular C‑terminal lectin domain. Distinct regions of TSP‑1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSP‑1 shares 95% and 99% amino acid sequence identity with human and rat TSP‑1, respectively. TSP‑1 counteracts the angiogenic, hypotensive, and anti‑thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4‑6). TSP‑1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF‑beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP‑1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP‑1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

References

Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
Laherty, C.D. et al. (1992) J. Biol. Chem. 267:3274.
Roberts, D.D. et al. (2012) Matrix Biol. 31:162.
Gupta, K. et al. (1999) Angiogenesis 3:147.
Kaur, S. et al. (2010) J. Biol. Chem. 285:38923.
Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79.
Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
Isenberg, J.S. et al. (2008) Blood 111:613.
Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775.
Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
Grimbert, P. et al. (2006) J. Immunol. 177:3534.
Kaur, S. et al. (2011) J. Biol. Chem. 286:14991.
Xu, J. et al. (2010) Nat. Neurosci. 13:22.
Eroglu, C. et al. (2009) Cell 139:380.
Blake, S.M. et al. (2008) EMBO J. 27:3069.
Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.

Entrez Gene IDs

7057 (Human); 21825 (Mouse)

Alternate Names

THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1

Related Research Areas

Citations for Recombinant Mouse Thrombospondin-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Critical role of thrombospondin-1 in promoting intestinal mucosal wound repair
Authors: Wilson, ZS;Raya-Sandino, A;Miranda, J;Fan, S;Brazil, JC;Quiros, M;Garcia-Hernandez, V;Liu, Q;Kim, CH;Hankenson, KD;Nusrat, A;Parkos, CA;
JCI insight
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay
Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons
Authors: Andries, L;Kancheva, D;Masin, L;Scheyltjens, I;Van Hove, H;De Vlaminck, K;Bergmans, S;Claes, M;De Groef, L;Moons, L;Movahedi, K;
Acta neuropathologica communications
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cells
Authors: KW Southerlan, Y Xu, DT Peters, X Wei, X Lin, Y Xiang, K Fei, LA Olivere, JM Morowitz, J Otto, Q Dai, CD Kontos, Y Diao
bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme
Authors: G Jacquemin, A Wurmser, M Huyghe, W Sun, Z Homayed, C Merle, M Perkins, F Qasrawi, S Richon, F Dingli, G Arras, D Loew, D Vignjevic, J Pannequin, S Fre
Elife, 2022-05-11;11(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice
Authors: C He, B Jiang, M Wang, P Ren, SI Murtada, AW Caulk, G Li, L Qin, R Assi, CJ Lovoulos, MA Schwartz, JD Humphrey, G Tellides
JCI Insight, 2022-02-08;7(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity
Authors: A Jeanne, T Sarazin, M Charlé, C Moali, C Fichel, C Boulagnon-, M Callewaert, MC Andry, E Diesis, F Delolme, D Rioult, S Dedieu
Cancers, 2021-10-07;13(19):.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR
Insulin inhibits inflammation-induced cone death in retinal detachment
Authors: JB Conart, G Blot, S Augustin, G Millet-Pue, C Roubeix, F Beguier, H Charles-Me, S Touhami, JA Sahel, JP Berrod, T Léveillard, X Guillonnea, C Delarasse, F Sennlaub
J Neuroinflammation, 2020-11-26;17(1):358.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Binding of omeprazole to protein targets identified by monoclonal antibodies
Authors: NMP Cartee, MM Wang
PLoS ONE, 2020-09-18;15(9):e0239464.
Species: Mouse
Sample Types: Reference Standard
Aging-associated changes in CD47 arrangement and interaction with thrombospondin-1 on red blood cells visualized by super-resolution imaging
Authors: F Wang, YH Liu, T Zhang, J Gao, Y Xu, GY Xie, WJ Zhao, H Wang, YG Yang
Aging Cell, 2020-08-31;0(0):e13224.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Capture
Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neuralï¿½Connections with Mouse Central Auditory Neurons Inï¿½Vitro via theï¿½?2?1 Receptor
Authors: Z Liu, Y Jiang, X Li, Z Hu
Stem Cell Reports, 2018-06-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Thrombospondin-1 signaling through CD47 inhibits cell cycle progression and induces senescence in endothelial cells
Cell Death Dis, 2016-09-08;7(9):e2368.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein.
Authors: Zheng Y, Zou F, Wang J, Yin G, Le V, Fei Z, Liu J
J Biol Chem, 2015-02-19;290(14):8975-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay