Recombinant Mouse TGF-beta RII/(mouse) Fc Chimera, CF Summary
Product Specifications
| Mouse TGF-beta RII (Ile24 - Asp184) Accession # Q62312 |
IEGRMDP | Mouse IgG2a (Glu98 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
1600-R2
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1600-R2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TGF-beta RII
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors (1).
The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.
Citations for Recombinant Mouse TGF-beta RII/(mouse) Fc Chimera, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury.
Proc Natl Acad Sci U S A, 2013-01-23;110(6):2324-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Therapeutic blockade of transforming growth factor beta fails to promote clearance of a persistent viral infection.
J. Virol., 2012-05-02;86(13):7060-71.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011-07-07;286(34):30034-46.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Combined Intratumoral Regulatory T-Cell Depletion and Transforming Growth Factor-beta Neutralization Induces Regression of Established AE17 Murine Mesothelioma Tumors.
Authors: Kissick HT, Ireland DJ, Beilharz MW
J. Interferon Cytokine Res., 2009-04-01;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
TLR4 enhances TGF-beta signaling and hepatic fibrosis.
Authors: Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF
Nat. Med., 2007-10-21;13(11):1324-32.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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