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Recombinant Mouse TGF-beta RII/(human) Fc Chimera, CF

Catalog #: 532-R2
4 Citations Datasheet
Catalog # Availability Size / Price Qty
532-R2-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TGF-beta RII/(human) Fc Chimera, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 10-70 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TGF-beta RII protein
Mouse TGF-beta RII
(Ile24-Asp184)
Accession # Q62312.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q62312.1
N-terminal Sequence
Analysis
Ile24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44.6 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions

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532-R2

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

532-R2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References
  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

Citations for Recombinant Mouse TGF-beta RII/(human) Fc Chimera, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Mechanisms of immune suppression exerted by regulatory T-cells in subcutaneous AE17 murine mesothelioma.
    Authors: Kissick HT, Ireland DJ, Greay SJ, Beilharz MW
    J. Interferon Cytokine Res., 2010-09-13;30(11):829-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Partial inhibition of integrin alpha(v)beta6 prevents pulmonary fibrosis without exacerbating inflammation.
    Authors: Horan GS, Wood S, Ona V, Li DJ, Lukashev ME, Weinreb PH, Simon KJ, Hahm K, Allaire NE, Rinaldi NJ, Goyal J, Feghali-Bostwick CA, Matteson EL, O'Hara C, Lafyatis R, Davis GS, Huang X, Sheppard D, Violette SM
    Am. J. Respir. Crit. Care Med., 2007-10-04;177(1):56-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling.
    Authors: Close JL, Gumuscu B, Reh TA
    Development, 2005-07-01;132(13):3015-26.
    Species: Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  4. Thrombospondin plays a vital role in the immune privilege of the eye.
    Authors: Zamiri P, Masli S, Kitaichi N, Taylor AW, Streilein JW
    Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):908-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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