Recombinant Mouse SPARC Protein, CF

Catalog #: 942-SP
6 Citations 2 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
942-SP-050

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Citations (6)

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Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SPARC Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED₅₀ for this effect is 0.6‑2.4 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse SPARC protein
Ala18-Ile302, with a C-terminal 10-His tag

Accession #

P07214

N-terminal Sequence
Analysis

Ala18

Predicted Molecular Mass

34 kDa

SDS-PAGE

42 kDa, reducing conditions

Product Datasheets

942-SP

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

942-SP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1 - 5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1 - 5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3 - 5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3 - 5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.

References

Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
Sage, H. et al. (1989) J. Cell Biol. 109:341.
Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
Hohenester, E. et al. (1997) EMBO J. 16:3778.
Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
Delany, A.M. et al. (2003) Endocrinology 144:2588.
Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Entrez Gene IDs

6678 (Human); 20692 (Mouse)

Alternate Names

Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC

Related Research Areas

Citations for Recombinant Mouse SPARC Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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SPARC promotes insulin secretion through down-regulation of RGS4 protein in pancreatic &beta cells
Authors: L Hu, F He, M Huang, Q Zhao, L Cheng, N Said, Z Zhou, F Liu, YS Dai
Sci Rep, 2020-10-16;10(1):17581.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
Oncogene, 2019-02-14;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Implication of SPARC in the modulation of the extracellular matrix and mitochondrial function in muscle cells
Authors: A Melouane, A Carbonell, M Yoshioka, J Puymirat, J St-Amand
PLoS ONE, 2018-02-08;13(2):e0192714.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
SPARC and GluA1-Containing AMPA Receptors Promote Neuronal Health Following CNS Injury
Authors: EV Jones, Y Bernardine, JG Zarruk, S Chierzi, KK Murai
Front Cell Neurosci, 2018-02-01;12(0):22.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
SPARC deficiency affects bone marrow stromal function, resulting in impaired B lymphopoiesis.
Authors: Luo Z, Zhou Y, Luo P, Zhao Q, Xiao N, Yu Y, Yan Q, Lu G, Cheng L
J Leukoc Biol, 2014-03-05;96(1):73-82.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Astrocytes control glutamate receptor levels at developing synapses through SPARC-beta-integrin interactions.
Authors: Jones EV, Bernardinelli Y, Tse YC, Chierzi S, Wong TP, Murai KK
J. Neurosci., 2011-03-16;31(11):4154-65.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay