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Recombinant Mouse RAGE Fc Chimera Protein, CF

Catalog #: 1179-RG
9 Citations Datasheet
Catalog # Availability Size / Price Qty
1179-RG-050
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Citations (9)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse RAGE Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. rmRAGE/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody-coated plate (0.5 µg/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA, Catalog # BT4127) with a linear range of 0.02-1 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse RAGE protein
Mouse RAGE
(Gln24 - Ala342)
Accession #O35444		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
O35444
N-terminal Sequence
Analysis
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60.5 kDa (monomer)
SDS-PAGE
80-85 kDa, reducing conditions

Product Datasheets

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1179-RG

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1179-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

References
  1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
  2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
  3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
  4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
  5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  7. Valencia, J.V. et al. (2004) Diabetes 53:743.
Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

Citations for Recombinant Mouse RAGE Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. sRAGE alleviates neutrophilic asthma by blocking HMGB1/RAGE signalling in airway dendritic cells
    Authors: F Zhang, X Su, G Huang, XF Xin, EH Cao, Y Shi, Y Song
    Sci Rep, 2017-10-27;7(1):14268.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  2. RAGE inhibition reduces acute lung injury in mice
    Authors: R Blondonnet, J Audard, C Belville, G Clairefond, J Lutz, D Bouvier, L Roszyk, C Gross, M Lavergne, M Fournet, L Blanchon, C Vachias, C Damon-Soub, V Sapin, JM Constantin, M Jabaudon
    Sci Rep, 2017-08-03;7(1):7208.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Dietary sugars, not lipids, drive hypothalamic inflammation
    Authors: Y Gao, M Bielohuby, T Fleming, GF Grabner, E Foppen, W Bernhard, M Guzmán-Rui, C Layritz, B Legutko, E Zinser, C García-Các, RM Buijs, SC Woods, A Kalsbeek, RJ Seeley, PP Nawroth, M Bidlingmai, MH Tschöp, CX Yi
    Mol Metab, 2017-06-20;6(8):897-908.
    Applications: Bioassay
  4. Regulation of RAGE ectodomain shedding and its role in cell function
    Authors: A Braley, T Kwak, J Jules, E Harja, R Landgraf, BI Hudson
    J Biol Chem, 2016-03-28;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
    Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
    PLoS ONE, 2015-04-07;10(4):e0123293.
    Species: Mouse
    Sample Types: Protein
    Applications: Binding Assay
  6. Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.
    Authors: Mizumoto S, Takahashi J, Sugahara K
    J. Biol. Chem., 2012-04-09;287(23):18985-94.
    Species: Mouse
    Sample Types: Carbohydrates, Whole Cells
    Applications: Flow Cytometry, Surface Plasmon Resonance
  7. Oxidative stress-associated rise of hepatic protein glycation increases inflammatory liver injury in uncoupling protein-2 deficient mice.
    Authors: Kuhla A, Hettwer C, Menger MD
    Lab. Invest., 2010-04-05;90(8):1189-98.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Receptor for advanced glycation end-products (RAGE) is an indicator of direct lung injury in models of experimental lung injury.
    Authors: Su X, Looney MR, Gupta N, Matthay MA
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-05-01;297(1):L1-5.
    Applications: ELISA (Standard)
  9. Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock.
    Authors: Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J
    Nat. Med., 2007-09-02;13(9):1042-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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