Recombinant Mouse Klotho (aa 35-982) Protein, CF

Catalog #: 1819-KL
26 Citations 3 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
1819-KL-050

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Citations (26)

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Reviews (3)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Klotho (aa 35-982) Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED₅₀ for this effect is 0.04-0.4 µg/mL in the presence of 1 µg/mL of Recombinant Human FGF‑23 (Catalog # 2604-FG) and 10 µg/mL of heparin.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Klotho protein
Ala35-Lys982, with a C-terminal 6-His tag

Accession #

O35082.2

N-terminal Sequence
Analysis

Ala35

Predicted Molecular Mass

109.5 kDa

SDS-PAGE

125-130 kDa, reducing conditions

Product Datasheets

1819-KL

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1819-KL

Formulation	Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87% and 87% aa identity with rat, human and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)₂D₃ (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10 - 12).

References

Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
Kuro-o, M. et al. (1997) Nature 390:45.
Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
Imura, A. et al. (2004) FEBS Lett. 565:143.
Kurosu, H. et al. (2005) Science 309:1829.
Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
Chang, Q. et al. (2005) Science 310:490.
Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
Liu, H. et al. (2007) Science 317:803.
Imura, A. et al. (2007) Science 316:1615.

Entrez Gene IDs

9365 (Human); 16591 (Mouse)

Alternate Names

EC 3.2.1; EC 3.2.1.31; KL; Klotho

Related Research Areas

Citations for Recombinant Mouse Klotho (aa 35-982) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Molecular Interaction of Soluble Klotho with FGF23 in the Pathobiology of Aortic Valve Lesions Induced by Chronic Kidney Disease
Authors: The, E;Zhai, Y;Yao, Q;Ao, L;Fullerton, DA;Meng, X;
International journal of biological sciences
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: In vivo assay
Klotho increases antioxidant defenses in astrocytes and ubiquitin-proteasome activity in neurons
Authors: Orellana, AM;Mazucanti, CH;Dos Anjos, LP;de Sá Lima, L;Kawamoto, EM;Scavone, C;
Scientific reports
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Neuroprotective action of alpha-Klotho against LPS-activated glia conditioned medium in primary neuronal culture
Authors: VW Nakao, CHY Mazucanti, L de Sá Lima, PS de Mello, NM de Souza P, PF Kinoshita, JA Leite, EM Kawamoto, C Scavone
Scientific Reports, 2022-11-07;12(1):18884.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Angiotensin II receptor type 1 blockade regulates Klotho expression to induce TSC2-deficient cell death
Authors: S Shrestha, E Adib, J Imani, DJ Aguiar, AM Lamattina, DD Tassew, EP Henske, MA Perrella, C Priolo, S El-Chemaly
The Journal of Biological Chemistry, 2022-10-08;0(0):102580.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture
Phosphate Metabolic Inhibition Contributes to Irradiation-Induced Myelosuppression through Dampening Hematopoietic Stem Cell Survival
Authors: Y Wu, W Liao, J Chen, C Liu, S Zhang, K Yu, X Wang, M Chen, S Wang, X Ran, Y Su, T Cheng, J Wang, C Du
Nutrients, 2022-08-18;14(16):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Renal Klotho and inorganic phosphate are extrinsic factors that antagonistically regulate hematopoietic stem cell maintenance
Authors: C Du, X Wang, Y Wu, W Liao, J Xiong, Y Zhu, C Liu, W Han, Y Wang, S Han, S Chen, Y Xu, S Wang, F Wang, K Yang, J Zhao, J Wang
Cell Reports, 2022-02-15;38(7):110392.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay
Klotho attenuated Doxorubicin-induced cardiomyopathy by alleviating Dynamin-related protein 1 - mediated mitochondrial dysfunction
Authors: X Zhuang, X Sun, H Zhou, S Zhang, X Zhong, X Xu, Y Guo, Z Xiong, M Liu, Y Lin, M Zhang, X Liao
Mechanisms of Ageing and Development, 2021-02-01;195(0):111442.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay
Centrally circulating &alpha-klotho inversely correlates with human obesity and modulates arcuate cell populations in mice
Authors: T Landry, P Li, D Shookster, Z Jiang, H Li, BT Laing, W Bunner, T Langton, Q Tong, H Huang
Molecular Metabolism, 2020-12-07;44(0):101136.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infants
Authors: S Batlahally, A Franklin, A Damianos, J Huang, P Chen, M Sharma, J Duara, D Keerthy, R Zambrano, LA Shehadeh, EC Martinez, MJ DeFreitas, S Kulandavel, CL Abitbol, M Freundlich, RM Kanashiro-, A Schmidt, M Benny, S Wu, KK Mestan, KC Young
Sci Rep, 2020-07-23;10(1):12368.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROS/P38 MAPK-Regulated Inflammatory Responses and Apoptosis
Authors: Z Zhang, Q Nian, G Chen, S Cui, Y Han, J Zhang
Oxid Med Cell Longev, 2020-05-13;2020(0):1854206.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Soluble Alpha-Klotho Alleviates Cardiac Fibrosis without Altering Cardiomyocytes Renewal
Authors: WY Chen
Int J Mol Sci, 2020-03-22;21(6):.
Species: Rat
Sample Types: cardiomyocyte
Applications: Bioassay

Local Klotho enhances neuronal progenitor proliferation in the adult hippocampus
Authors: F Salech, L Varela-Nal, SB Arredondo, DB Bustamante, GA Andaur, R Cisneros, DP Ponce, P Ayala, NC Inestrosa, JL Valdés, MI Behrens, A Couve
J. Gerontol. A Biol. Sci. Med. Sci., 2019-06-18;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice
Authors: Z Rao, T Landry, P Li, W Bunner, BT Laing, Y Yuan, H Huang
Heliyon, 2019-04-24;5(4):e01494.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Klotho controls the brain-immune system interface in the choroid plexus
Authors: L Zhu, LR Stein, D Kim, K Ho, GQ Yu, L Zhan, TE Larsson, L Mucke
Proc. Natl. Acad. Sci. U.S.A., 2018-11-09;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Klotho suppresses colorectal cancer through modulation of the unfolded protein response
Authors: T Arbel Rubi, S Shahmoon, E Zigmond, T Etan, K Merenbakh-, M Pasmanik-C, G Har-Zahav, I Barshack, GW Vainer, N Skalka, R Rosin-Arbe, C Varol, T Rubinek, I Wolf
Oncogene, 2018-09-19;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Soluble Klotho causes hypomineralization in Klotho-deficient mice
Authors: T Minamizaki, Y Konishi, K Sakurai, H Yoshioka, JE Aubin, K Kozai, Y Yoshiko
J. Endocrinol., 2018-04-09;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury
Authors: Y Qian, X Guo, L Che, X Guan, B Wu, R Lu, M Zhu, H Pang, Y Yan, Z Ni, L Gu
Cell. Physiol. Biochem., 2018-03-10;45(6):2268-2282.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Activity-dependent neuronal Klotho enhances astrocytic aerobic glycolysis
Authors: CH Mazucanti, EM Kawamoto, MP Mattson, C Scavone, S Camandola
J. Cereb. Blood Flow Metab., 2018-03-01;0(0):271678X187627.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Peripheral Elevation of a Klotho Fragment Enhances Brain Function and Resilience in Young, Aging, and ?-Synuclein Transgenic Mice
Authors: J Leon, AJ Moreno, BI Garay, RJ Chalkley, AL Burlingame, D Wang, DB Dubal
Cell Rep, 2017-08-08;20(6):1360-1371.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Klotho enhances FoxO3-mediated manganese superoxide dismutase expression by negatively regulating PI3K/AKT pathway during tacrolimus-induced oxidative stress
Authors: SW Lim, L Jin, K Luo, J Jin, YJ Shin, SY Hong, CW Yang
Cell Death Dis, 2017-08-03;8(8):e2972.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Inhibition of age-related therapy resistance in melanoma by rosiglitazone-mediated induction of Klotho
Authors: R Behera, A Kaur, MR Webster, S Kim, A Ndoye, CH Kugel, GM Alicea, JX Wang, K Ghosh, PF Cheng, S Lisanti, K Marchbank, V Dang, MP Levesque, R Dummer, X Xu, M Heryn, AE Aplin, A Roesch, MC Caino, DC Altieri, AT Weeraratna
Clin. Cancer Res, 2017-02-23;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

FGF23 promotes renal calcium reabsorption through the TRPV5 channel.
Authors: Andrukhova O, Smorodchenko A, Egerbacher M, Streicher C, Zeitz U, Goetz R, Shalhoub V, Mohammadi M, Pohl E, Lanske B, Erben R
EMBO J, 2014-01-16;33(3):229-46.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Klotho deficiency disrupts hematopoietic stem cell development and erythropoiesis.
Authors: Vadakke Madathil S, Coe L, Casu C, Sitara D
Am J Pathol, 2014-01-08;184(3):827-41.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Klotho suppresses cardiomyocyte apoptosis in mice with stress-induced cardiac injury via downregulation of endoplasmic reticulum stress.
Authors: Song, Shuang, Gao, Pan, Xiao, Hang, Xu, Yan, Si, Liang Yi
PLoS ONE, 2013-12-10;8(12):e82968.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

FGF23 suppresses chondrocyte proliferation in the presence of soluble alpha-Klotho both in vitro and in vivo.
Authors: Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, Ohata Y, Ozono K, Michigami T
J Biol Chem, 2012-12-12;288(4):2414-27.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
Kidney Int, 2012-08-29;82(12):1261-70.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay