Recombinant Mouse Insulin R/CD220 Protein, CF Summary
Product Specifications
His28-Ser748 ( alpha subunit) & Ser753-Lys946, with a C-terminal 6-His tag ( beta subunit)
Analysis
Product Datasheets
7544-MR
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7544-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Insulin R/CD220
The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1382 amino acid (aa) mouse Insulin R preproprotein is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-748), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 753-1372) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The Mouse Insulin R ECD shares 96%, 99%, 94% and 94% aa sequence identity with human, rat, equine and canine Insulin R, respectively. Insulin R may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Consistent with mice deleted for Insulin R, mutations in the human Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). The R&D Systems mouse Insulin R consists of the entire ECD and is a pre-receptor that does not contain bound insulin.
- Nakae, J. et al. (2001) Endoc. Rev. 22:818.
- Sciacca, L. et al. (2003) Endocrinology 144:2650.
- Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
- Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
- Sacco, A. et al. (2009) Endocrinology 150:3594.
- Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
- Musso, C. et al. (2004) Medicine (Baltimore) 83:209.
Citations for Recombinant Mouse Insulin R/CD220 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Identification of lamprey variable lymphocyte receptors that target the brain vasculature
Authors: JM Lajoie, ME Katt, EA Waters, BR Herrin, EV Shusta
Scientific Reports, 2022-04-11;12(1):6044.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity
Authors: JG Labombarde, MR Pillai, M Wehenkel, CY Lin, R Keating, SA Brown, JC Crawford, DC Brice, AH Castellaw, AH Mandarano, CS Guy, JR Mejia, CD Lewis, TC Chang, CM Oshansky, SS Wong, RJ Webby, M Yan, QZ Li, TN Marion, PG Thomas, MA McGargill
Cell Reports, 2022-03-08;38(10):110482.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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