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Recombinant Mouse IL-17A/F Heterodimer Protein

Catalog #: 5390-IL
3 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
5390-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5390-IL-025
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-17A/F Heterodimer Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED50 for this effect is 0.075-0.375 μg/mL.
Source
E. coli-derived mouse IL-17A/F Heterodimer protein
Mouse IL-17A
(Thr22 - Ala158)
Accession # Q62386
Mouse IL-17F
(Arg21 - Ala153, with an N-terminal Met)
Accession # AAM77568
N-terminus C-terminus
Accession #
Q62386 (IL-17A) & AAM77568 (IL-17F)
N-terminal Sequence
Analysis
Thr22 (IL-17A) & Met (IL-17F)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
14.9 kDa (IL-17A) & 15.5 kDa (IL-17F), reducing conditions

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5390-IL (with carrier)

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5390-IL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5390-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5390-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17A/F Heterodimer

IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1). Members of this family demonstrate a structural motif termed a cysteine-knot that also characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2 - 4). Mouse IL-17A is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 133 aa mature region. The mature chain contains one potential site of N-linked glycosylation. Mouse IL-17F is synthesized as a 161 aa precursor. Like IL-17A, IL-17F contains one potential site of N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. Mouse IL-17A and IL-17F are 61% and 56% identical to human IL-17A and IL-17F on the aa level, respectively. IL-17A/F and the IL-17A and IL-17F homodimers are produced by an activated subset of CD4+ T cells, termed Th17 (1, 5 - 7). IL-23 drives Th17 lymphocytes to produce these cytokines (5, 8 - 9). Studies have identified that the widely expressed receptors IL-17RA and IL-17RC form a heterodimer for the binding of IL-17A and IL-17F, and IL-17A/F (5, 10 - 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (6).

References
  1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  2. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  3. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  4. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  5. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
  6. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  7. Ouyang, W. et al. (2008) Immunity 28:454.
  8. Steinman, L. (2007) Nat. Med. 13:139.
  9. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  10. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  11. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Long Name
Interleukin 17 A/F
Alternate Names
CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A

Citations for Recombinant Mouse IL-17A/F Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protection against Staphylococcus aureus Colonization and Infection by B- and T-Cell-Mediated Mechanisms
    Authors: F Zhang, O Ledue, M Jun, C Goulart, R Malley, YJ Lu
    MBio, 2018-10-16;9(5):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function
    Proc Natl Acad Sci USA, 2016-09-26;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Role of IL-17A in neutrophil recruitment and hepatic injury after warm ischemia-reperfusion mice.
    Authors: Kono H, Fujii H, Ogiku M, Hosomura N, Amemiya H, Tsuchiya M, Hara M
    J. Immunol., 2011-09-26;187(9):4818-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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