Recombinant Mouse IL-17A (CHO-expressed) Protein

Catalog #: 7956-ML
5 Citations 1 Review Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
7956-ML-025/CF
7956-ML-010/CF
7956-ML-100/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7956-ML-025

R&D Systems Recombinant Proteins and Enzymes

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All IL-17/IL-17A Proteins and Enzymes

Product Details

Citations (5)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-17A (CHO-expressed) Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.25-1.25 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse IL-17/IL-17A protein
Ala26-Ala158

Accession #

Q62386

N-terminal Sequence
Analysis

Ala26

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

15 kDa (monomer)

SDS-PAGE

15 -22 kDa, reducing conditions

Product Datasheets

7956-ML (with carrier)

Product Datasheet

7956-ML/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7956-ML

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7956-ML/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL‑17A shares 61% and 89% amino acid sequence identity with human and rat IL‑17A, respectively (3, 4). IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (17, 18).

References

Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
Yao, Z. et al. (1996) Gene 168:223.
Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Rong, Z. et al. (2009) Cell Res. 19:208.
Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
Hsu, H. et al. (2008) Nat. Immunol. 9:166.
Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
Wang, L. et al. (2009) J. Exp. Med. 206:1457.
Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Related Research Areas

Citations for Recombinant Mouse IL-17A (CHO-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Glia-derived adenosine in the ventral hippocampus drives pain-related anxiodepression in a mouse model resembling trigeminal neuralgia
Authors: Lv, XJ;Lv, SS;Wang, GH;Chang, Y;Cai, YQ;Liu, HZ;Xu, GZ;Xu, WD;Zhang, YQ;
Brain, behavior, and immunity
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Bioassay
Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis
Authors: Wayne, CR;Bremner, L;Faust, TE;Dur�n-Laforet, V;Ampatey, N;Ho, SJ;Feinberg, PA;Arvanitis, P;Ciric, B;Ruan, C;Elyaman, W;Delaney, SL;Vargas, WS;Swedo, S;Menon, V;Schafer, DP;Cutforth, T;Agalliu, D;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
Nature Immunology, 2022-10-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway
Authors: EM Byrne, M Llorián-Sa, M Tang, A Margariti, M Chen, H Xu
Biomedicines, 2021-07-16;9(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy
Authors: Y Wang, S Gao, S Gao, N Li, B Xie, X Shen
Cell & bioscience, 2021-05-01;11(1):82.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay