Home / IL-1 RI / Recombinant Mouse IL-1 RI Fc Chimera Protein, CF

Recombinant Mouse IL-1 RI Fc Chimera Protein, CF

Catalog #: 771-MR
3 Citations Datasheet
Catalog # Availability Size / Price Qty
771-MR-100
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-1 RI Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. Approximately 0.005‑0.015 µg/mL of IL-1 sRI/Fc Chimera will inhibit 50% of the biological response due to 50 pg/mL of rmIL-1a.
Source
Mouse myeloma cell line, NS0-derived mouse IL-1 RI protein
Mouse IL-1 RI
(Leu20 - Lys338)
Accession # P13504		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
P13504
N-terminal Sequence
Analysis
Leu20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
63.8 kDa (monomer)
SDS-PAGE
95-110 kDa, reducing conditions

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771-MR

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

771-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains.

An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor I
Entrez Gene IDs
3554 (Human); 16177 (Mouse)
Alternate Names
CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

Citations for Recombinant Mouse IL-1 RI Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet.
    Authors: Grayson BE, Levasseur PR, Williams SM, Smith MS, Marks DL, Grove KL
    Endocrinology, 2010-02-22;151(4):1622-32.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Whole Tissue
    Applications: Antibody Absorption
  2. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.
    Authors: Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA
    J. Immunol., 2007-08-15;179(4):2551-5.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  3. Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
    Authors: O&amp;apos;Connor JC, Sherry CL, Guest CB, Freund GG
    J. Immunol., 2007-06-01;178(11):6886-93.
    Applications: ELISA (Standard)

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