Recombinant Mouse IL-1 alpha/IL-1F1 Protein Summary
Product Specifications
Ser6-Ser161
Analysis
Product Datasheets
400-ML (with carrier)
400-ML/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
400-ML
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
400-ML/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 3-7 pg/mL.
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1 μg/lane of Recombinant Mouse IL-1 alpha /IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Background: IL-1 alpha/IL-1F1
IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.
Citations for Recombinant Mouse IL-1 alpha/IL-1F1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth
Authors: Donahue, KL;Watkoske, HR;Kadiyala, P;Du, W;Brown, K;Scales, MK;Elhossiny, AM;Espinoza, CE;Lasse Opsahl, EL;Griffith, BD;Wen, Y;Sun, L;Velez-Delgado, A;Renollet, NM;Morales, J;Nedzesky, NM;Baliira, RK;Menjivar, RE;Medina-Cabrera, PI;Rao, A;Allen, B;Shi, J;Frankel, TL;Carpenter, ES;Bednar, F;Zhang, Y;Pasca di Magliano, M;
Cancer discovery
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis
Authors: LaMarche, NM;Hegde, S;Park, MD;Maier, BB;Troncoso, L;Le Berichel, J;Hamon, P;Belabed, M;Mattiuz, R;Hennequin, C;Chin, T;Reid, AM;Reyes-Torres, I;Nemeth, E;Zhang, R;Olson, OC;Doroshow, DB;Rohs, NC;Gomez, JE;Veluswamy, R;Hall, N;Venturini, N;Ginhoux, F;Liu, Z;Buckup, M;Figueiredo, I;Roudko, V;Miyake, K;Karasuyama, H;Gonzalez-Kozlova, E;Gnjatic, S;Passegué, E;Kim-Schulze, S;Brown, BD;Hirsch, FR;Kim, BS;Marron, TU;Merad, M;
Nature
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TPL2 kinase activity regulates microglial inflammatory responses and promotes neurodegeneration in tauopathy mice
Authors: Wang, Y;Wu, T;Tsai, MC;Rezzonico, MG;Abdel-Haleem, AM;Xie, L;Gandham, VD;Ngu, H;Stark, K;Glock, C;Xu, D;Foreman, O;Friedman, BA;Sheng, M;Hanson, JE;
eLife
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Augmented secretion of IL-1&alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells
Authors: A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh
Journal of oral biosciences, 2021-06-18;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke
Authors: KE Salmeron, ME Maniskas, DN Edwards, R Wong, I Rajkovic, A Trout, AA Rahman, S Hamilton, JF Fraser, E Pinteaux, GJ Bix
J Neuroinflammation, 2019-11-14;16(1):222.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Caspase-11-dependent IL-1α release boosts Th17 immunity against Paracoccidioides brasiliensis
Authors: N Ketelut-Ca, COS Souza, L Benevides, LG Gardinassi, MC Silva, LA Tavares, DS Zamboni, JS Silva
PLoS Pathog., 2019-08-19;15(8):e1007990.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis
Authors: H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson
J. Immunol., 2019-07-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation
Authors: X Liu, Y Sun, Y Zheng, M Zhang, X Jin, K Kang, Y Wang, S Li, H Zhang, Q Zhao, S Zhang, J Wu, B Yu
Cell. Physiol. Biochem., 2018-09-07;49(3):1180-1196.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction
Authors: PC Duncker, JS Stoolman, AK Huber, BM Segal
J. Immunol., 2017-12-29;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
LZTFL1 Upregulated by All-Trans Retinoic Acid during CD4+ T Cell Activation Enhances IL-5 Production.
Authors: Jiang H, Promchan K, Lin B, Lockett S, Chen D, Marshall H, Badralmaa Y, Natarajan V
J Immunol, 2015-12-23;196(3):1081-90.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis.
Authors: Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X
J Biol Chem, 2015-08-14;290(39):23738-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Secreted IL-1alpha promotes T-cell activation and expansion of CD11b(+) Gr1(+) cells in carbon tetrachloride-induced liver injury in mice.
Authors: Lin D, Lei L, Zhang Y, Hu B, Bao G, Liu Y, Song Y, Liu C, Wu Y, Zhao L, Yu X, Liu H
Eur J Immunol, 2015-04-29;45(7):2084-98.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M.
Authors: Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F
PLoS ONE, 2014-07-10;9(7):e101937.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle.
Authors: Chen D, Goswami C, Burnett R, Anjanappa M, Bhat-Nakshatri P, Muller W, Nakshatri H
Cancer Res, 2014-06-30;74(16):4270-81.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Distinctive subdomains in the resorbing surface of osteoclasts.
Authors: Szewczyk K, Fuller K, Chambers T
PLoS ONE, 2013-03-21;8(3):e60285.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-1beta promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation.
Authors: Jayaraman P, Sada-Ovalle I, Nishimura T, Anderson A, Kuchroo V, Remold H, Behar S
J Immunol, 2013-03-13;190(8):4196-204.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
J. Exp. Med., 2012-07-16;209(8):1505-17.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
J. Virol., 2010-09-29;84(24):12703-12.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
J. Immunol., 2010-03-24;184(0):5263-70.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells.
Authors: Dienz O, Eaton SM, Bond JP, Neveu W, Moquin D, Noubade R, Briso EM, Charland C, Leonard WJ, Ciliberto G, Teuscher C, Haynes L, Rincon M
J. Exp. Med., 2009-01-12;206(1):69-78.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.
Authors: Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA
J. Immunol., 2007-08-15;179(4):2551-5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Protection of CD8+ T cells from activation-induced cell death by IL-18.
Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
J. Leukoc. Biol., 2007-03-30;82(1):142-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-1 mediates TNF-induced osteoclastogenesis.
Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
J. Clin. Invest., 2005-02-01;115(2):282-90.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Loss of transforming growth factor counteraction on interleukin 1 mediated effects in cartilage of old mice.
Authors: Scharstuhl A, van Beuningen HM, Vitters EL, van der Kraan PM, van den Berg WB
Ann. Rheum. Dis., 2002-12-01;61(12):1095-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
CD40 regulates the processing of NF-kappaB2 p100 to p52.
Authors: Coope HJ, Atkinson PG, Huhse B, Belich M, Janzen J, Holman MJ, Klaus GG, Johnston LH, Ley SC
EMBO J., 2002-10-15;21(20):5375-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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