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Recombinant Mouse IGFBP-1 Protein, CF

Catalog #: 1588-B1
3 Citations Datasheet
Catalog # Availability Size / Price Qty
1588-B1-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IGFBP-1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.25-1.5 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IGF-I.
Source
Mouse myeloma cell line, NS0-derived mouse IGFBP-1 protein
Ala26-Asn272
Accession #
P47876
N-terminal Sequence
Analysis
Ala26
Predicted Molecular Mass
27 kDa
SDS-PAGE
34 kDa, reducing conditions

Product Datasheets

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1588-B1

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1588-B1

Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: IGFBP-1

The insulin-like growth factor binding protein (IGFBP) family consists of six structurally related proteins that bind IGF with high affinity (1, 2). These proteins share conserved cysteine-rich N- and C- terminal regions that participate in IGF binding. IGFBPs regulate the bioavailability of IGFs and modulate their biological activities, both positively and negatively. Some IGFBPs also have intrinsic bioactivity that is IGF-independent. Post-transitional modifications of the IGFBPs, including glycosylation, phosphorylation and proteolysis, influence IGF binding affinities and tissue localization, affecting both the IGF-dependent and independent functions (1, 2).

Mouse IGFBP-1 cDNA encodes a 272 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide (3). Mature mouse IGFBP-1 contains potential phosphorylation and proteolytic cleavage sites, an Arg-Gly-Asp (RGD) integrin receptor recognition sequence, but lacks potential N-linked glycosylation sites.
IGFBP-1 binds equally well to IGF-I and IGF-II. Phosphorylation of human IGFBP-1, but not rat IGFBP-1, increases IGF affinity. Mouse IGFBP-1 shares 67% and 93% aa sequence identity with human and rat IGFBP-1, respectively. IGFBP-1 is expressed in liver, decidua, and kidneys and is the most abundant IGFBP in amniotic fluid. Hepatic production of IGFBP-1 is down-regulated by insulin and up-regulated by glucocorticoids. Circulating IGFBP-1 levels are elevated under various catabolic conditions including bacterial or viral infections, trauma and diabetes (4). IGFBP-1 has been shown to either potentiate or inhibit the activities of IGF in a variety of cells. IGFBP-1, through its RDG motif, also interacts with alpha 5 beta 1 intergrin to stimulate changes in cell adhesion and migration in the absence of IGFs (1, 2).

References
  1. Firth, S.M. and R.C. Baxter (2002) Endocrine Reviews 23:824.
  2. Rajaram, S. et al. (1997) Endocrine Reviews 18:801.
  3. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
  4. Lang, C.H. et al. (2003) Endocrinology 144:3922.
Long Name
Insulin-like Growth Factor Binding Protein 1
Entrez Gene IDs
3484 (Human); 16006 (Mouse)
Alternate Names
alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP

Citations for Recombinant Mouse IGFBP-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IGFBP1 increases ?-cell regeneration by promoting ?- to ?-cell transdifferentiation
    EMBO J, 2016-08-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Endocrine actions of myostatin: systemic regulation of the IGF and IGF binding protein axis.
    Authors: Williams NG, Interlichia JP, Jackson MF
    Endocrinology, 2010-12-08;152(1):172-80.
    Applications: ELISA (Standard)
  3. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010-10-06;151(12):5741-50.
    Applications: ELISA (Standard)

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