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Recombinant Mouse GDF-15 (CHO-expressed) Protein, CF

Catalog #: 10596-GD Datasheet
Catalog # Availability Size / Price Qty
10596-GD-025
Recombinant Mouse GDF‑15 (CHO-expressed) Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GDF-15 (CHO-expressed) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Mouse GDF-15 (CHO-expressed) (Catalog # 10596-GD) binds to Recombinant Mouse GFR alpha -like Fc Chimera (Catalog # 9844-GR) with an ED50 of 0.500-5.00 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse GDF-15 protein
Ser189-Ala303
Accession #
Q9Z0J7.2
N-terminal Sequence
Analysis
Ser189
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa
SDS-PAGE
11-13 kDa, under reducing conditions,

Product Datasheets

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10596-GD

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10596-GD

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

In a functional ELISA, Recombinant Mouse GDF-15 (CHO-expressed) (Catalog # 10596-GD) binds to Recombinant Mouse GFR alpha -like Fc Chimera (9844-GR) with an ED50 of 0.500-5.00 ng/mL.

Bioactivity View Larger

Recombinant Mouse GDF-15 (Catalog # 10596-GD) activates SRE-SEAP reporter in HEK293 human embryonic kidney cells transfected with human c-Ret and human GFRAL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse GDF-15 (CHO-expressed) Protein (Catalog # 10596-GD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 11-13 kDa and 22-26 kDa, respectively.

Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similarly to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature mouse GDF-15 shares 59.6% and 91.9% amino acid sequence similarity with human and rat GDF-15, respectively. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early‑stage endochonadal bone formation, and promotion of neuronal survival. GDF-15 is the functional ligand for the receptor GFRAL, facilitating weight-loss functions of the protein through c-Ret downstream signaling. GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (8-10).

References
  1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  2. Böttner, M. et al. (1999) Gene 237:105.
  3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
  4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.
  8. Mullican, S. et al. (2017) Nat. Med 23:1150.
  9. Yang, L. et al. (2017) Nat. Med 23:1158.
  10. Emmerson, P. et al. (2017) Nat. Med 23:1215.
Long Name
Growth Differentiation Factor 15
Entrez Gene IDs
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

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