Recombinant Mouse FGFR4 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse FGF R4 (Leu19-Asp366) Accession # Q03142 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
2265-FR
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2265-FR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: FGFR4
Fibroblast growth factor receptor 4 (FGF R4), also known as CD334, is a 110 kDa glycosylated transmembrane receptor tyrosine kinase (1). Mature mouse FGF R4 consists of a 350 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 412 aa cytoplasmic domain that contains the tyrosine kinase domain (2). Within the ECD, mouse FGF R4 shares 90% and 96% aa sequence identity with human and rat FGF R4, respectively. Alternate splicing generates additional isoforms that have deletions within or C-terminal to the kinase domain (3, 4). FGF R4 is widely expressed during embryonic development and in adult liver, kidney, and lung (2, 5, 6, 7). It binds FGF acidic, FGF basic, FGF-8, -15, and -19 (6, 8‑12). FGF R4 associates with beta-Klotho and sulfated glycosaminoglycans, and these interactions increase the affinity of FGF R4 for its ligands as well as its signaling capacity (7, 9, 12). FGF-19 induced signaling through FGF R4 is important for the regulation of bile acid synthesis and lipid and glucose homeostasis (10, 13). FGF R4 supports glucose tolerance and insulin sensitivity and protects against hyperlipidemia (13). It is down‑regulated in the liver during fasting and is up‑regulated by insulin (10). It can exert either proliferative or apoptotic effects on hepatocytes (7, 11). FGF R4 signaling is additionally required for skeletal muscle development in limbs (4, 6). FGF R4 interacts in cis with cell surface MMP-14, leading to down‑regulation of both proteins (14). In contrast, the Arg388 variant of FGF R4, which is associated with tumor progression in human cancer, is activated and stabilized by MMP-14 (14, 15).
- Haugsten, E.M. et al. (2010) Mol. Cancer Res. 8:1439.
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- van Heumen, W.R.A. et al. (1999) IUBMB Life 48:73.
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- Shin, D.J. and T.F. Osborne (2009) J. Biol. Chem. 284:11110.
- Wu, X. et al. (2010) J. Biol. Chem. 285:5165.
- Nakamura, M. et al. (2011) J. Biol. Chem. 286:26418.
- Huang, X. et al. (2007) Diabetes 56:2501.
- Sugiyama, N. et al. (2010) Proc. Natl. Acad. Sci. 107:15786.
- Bange, J. et al. (2002) Cancer Res. 62:840.
Citation for Recombinant Mouse FGFR4 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Pre-clinical development of U3-1784, a novel FGFR4 antibody against cancer, and avoidance of its on-target toxicity
Authors: R Bartz, K Fukuchi, T Ohtsuka, T Lange, K Gruner, I Watanabe, S Hayashi, Y Oda, R Kawaida, H Komori, Y Kashimoto, P Wirtz, JA Mayer, M Redondo-Mü, S Saito, M Takahashi, H Hanzawa, E Imai, A Martinez, M Hanai, D Häussinger, RW Chapman, T Agatsuma, J Bange, R Abraham
Mol. Cancer Ther., 2019-07-26;0(0):.
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA Capture
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