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Recombinant Mouse FGF-4 Protein

Catalog #: 5846-F4
8 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
5846-F4-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5846-F4-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (8)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FGF-4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.75-3.75 ng/mL.
Source
E. coli-derived mouse FGF-4 protein
Ala30-Leu202
Accession #
NP_034332
N-terminal Sequence
Analysis
Ala30
Predicted Molecular Mass
19.0 kDa
SDS-PAGE
19 kDa, reducing conditions     

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5846-F4 (with carrier)

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5846-F4/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5846-F4

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5846-F4/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-4

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The mouse FGF-4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3 ‑ 5). Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6 ‑ 9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10 ‑ 12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

References
  1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
  3. Niswander, L. and G.R. Martin (1992) Development 114:755.
  4. Feldman, B. et al. (1995) Science 267:246.
  5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
  6. Sun, X. et al. (2002) Nature 418:501.
  7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
  8. Yu, K and D.M. Ornitz (2008) Development 135:483.
  9. Mariani, F.V. et al. (2008) Nature 453:401.
  10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
  11. Kunath, T. et al. (2007) Development 134:2895.
  12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
  13. Hajitou, A. et al. (1998) Oncogene 17:2059.
  14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.
Long Name
Fibroblast Growth Factor 4
Entrez Gene IDs
2249 (Human); 14175 (Mouse); 116499 (Rat)
Alternate Names
FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST

Citations for Recombinant Mouse FGF-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
    Authors: M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G
    Nature Cell Biology, 2022-09-13;24(9):1341-1349.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Mechanism of paracrine communications between hepatic progenitor cells and endothelial cells
    Authors: S Lee, R Karns, S Shin
    Cellular Signalling, 2022-08-30;100(0):110458.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Epiblast inducers capture mouse trophectoderm stem cells in�vitro and pattern blastoids for implantation in utero
    Authors: J Seong, J Frias-Alde, V Holzmann, H Kagawa, G Sestini, H Heidari Kh, Y Scholte Op, M Kip, SJ Pradhan, L Verwegen, J Vivié, L Li, A Alemany, J Korving, F Darmis, A van Oudena, D Ten Berge, N Geijsen, NC Rivron
    Cell Stem Cell, 2022-07-07;29(7):1102-1118.e8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. TRF2-independent chromosome end protection during pluripotency
    Authors: P Ruis, D Van Ly, V Borel, GR Kafer, A McCarthy, S Howell, R Blassberg, AP Snijders, J Briscoe, KK Niakan, P Marzec, AJ Cesare, SJ Boulton
    Nature, 2020-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes
    Authors: S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme
    Sci Rep, 2019-06-11;9(1):8470.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. 3D niche microarrays for systems-level analyses of cell fate.
    Authors: Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M
    Nat Commun, 2014-07-14;5(0):4324.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst.
    Authors: Le Bin G, Munoz-Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W, Etienne-Dumeau C, Grabole N, Mulas C, Niwa H, Hadjantonakis A, Nichols J
    Development, 2014-02-06;141(5):1001-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. FGF23 induces left ventricular hypertrophy.
    Authors: Faul C, Amaral AP, Oskouei B, Hu MC, Sloan A, Isakova T, Gutierrez OM, Aguillon-Prada R, Lincoln J, Hare JM, Mundel P, Morales A, Scialla J, Fischer M, Soliman EZ, Chen J, Go AS, Rosas SE, Nessel L, Townsend RR, Feldman HI, St John Sutton M, Ojo A, Gadegbeku C, Di Marco GS, Reuter S, Kentrup D, Tiemann K, Brand M, Hill JA, Moe OW, Kuro-o M, Kusek JW, Keane MG, Wolf M
    J. Clin. Invest., 2011-10-10;121(11):4393-408.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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