Recombinant Mouse FGF-4 (aa 67-202) Protein Summary
Product Specifications
Ser67-Leu202
Analysis
Product Datasheets
7486-F4 (with carrier)
7486-F4/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7486-F4
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mLin sterile, deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
7486-F4/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile, deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: FGF-4
FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The mouse FGF-4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF‑4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).
- Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
- Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
- Niswander, L. and G.R. Martin (1992) Development 114:755.
- Feldman, B. et al. (1995) Science 267:246.
- Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
- Sun, X. et al. (2002) Nature 418:501.
- Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
- Yu, K and D.M. Ornitz (2008) Development 135:483.
- Mariani, F.V. et al. (2008) Nature 453:401.
- Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
- Kunath, T. et al. (2007) Development 134:2895.
- Mayshar, Y. et al. (2008) Stem Cells 26:767.
- Hajitou, A. et al. (1998) Oncogene 17:2059.
- Flynn, A. and T. O’Brien (2008) IDrugs 11:283.
Citations for Recombinant Mouse FGF-4 (aa 67-202) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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A single-cell transcriptome atlas of human euploid and aneuploid blastocysts
Authors: Wang, S;Leng, L;Wang, Q;Gu, Y;Li, J;An, Y;Deng, Q;Xie, P;Cheng, C;Chen, X;Zhou, Q;Lu, J;Chen, F;Liu, L;Yang, H;Wang, J;Xu, X;Hou, Y;Gong, F;Hu, L;Lu, G;Shang, Z;Lin, G;
Nature genetics
Species: Human
Sample Types: Embryo
Applications: Bioassay -
Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro
Authors: Lau, KYC;Amadei, G;Zernicka-Goetz, M;
Nature protocols
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Macroscopic quorum sensing sustains differentiating embryonic stem cells
Authors: H Daneshpour, P van den Be, CH Chao, TG Fazzio, H Youk
Nature Chemical Biology, 2023-01-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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