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Recombinant Mouse EphB4 Fc Chimera Protein, CF

Catalog #: 446-B4
26 Citations Datasheet
Catalog # Availability Size / Price Qty
446-B4-200
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Recombinant Mouse EphB4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphB4 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin‑B2 Fc Chimera (Catalog # 496-EB) with a linear range of 0.078-5 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse EphB4 protein
Mouse EphB4
(Leu16-Ala539)
Accession # P54761		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P54761
N-terminal Sequence
Analysis
Leu16
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.2 kDa (monomer)
SDS-PAGE
110 kDa, reducing conditions

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446-B4

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

446-B4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin-B2 andephrin-B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name
Eph Receptor B4
Entrez Gene IDs
2050 (Human); 13846 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

Citations for Recombinant Mouse EphB4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Eph-ephrin signaling couples endothelial cell sorting and arterial specification
    Authors: Stewen, J;Kruse, K;Godoi-Filip, AT;Zenia, ;Jeong, HW;Adams, S;Berkenfeld, F;Stehling, M;Red-Horse, K;Adams, RH;Pitulescu, ME;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
    Authors: S Kohara, K Ogawa
    Biomedicines, 2022-12-12;10(12):.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Bioassay
  3. EphrinB2 and GRIP1 stabilize mushroom spines during denervation-induced homeostatic plasticity
    Authors: D Bissen, MK Kracht, F Foss, J Hofmann, A Acker-Palm
    Cell Reports, 2021-03-30;34(13):108923.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  4. PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival
    Authors: Y Yoon, G Voloudakis, N Doran, E Zhang, C Dimovasili, L Chen, Z Shao, S Darmanis, C Tang, J Tang, VX Wang, PR Hof, NK Robakis, A Georgakopo
    Mol. Psychiatry, 2020-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo
    Authors: YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao
    Oxid Med Cell Longev, 2020-06-05;2020(0):1404915.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling
    Authors: W Wang, C Yuan, T Geng, Y Liu, S Zhu, C Zhang, Z Liu, P Wang
    Stem Cell Res Ther, 2020-01-29;11(1):40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Authors: E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm
    Elife, 2019-12-23;8(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD
    Authors: A Grandi, I Zini, S Palese, C Giorgio, M Tognolini, F Marchesani, S Bruno, L Flammini, AM Cantoni, R Castelli, A Lodola, A Fusari, E Barocelli, S Bertoni
    Front Pharmacol, 2019-06-17;10(0):691.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  9. Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart
    Authors: D Herrero, S Cañón, G Albericio, RM Carmona, S Aguilar, S Mañes, A Bernad
    Redox Biol, 2019-03-04;22(0):101156.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  10. TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration
    Authors: J Yoon, YS Hwang, M Lee, J Sun, HJ Cho, L Knapik, IO Daar
    Nat Commun, 2018-08-28;9(1):3491.
    Species: Xenopus
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling
    Authors: JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim
    Exp. Mol. Med., 2018-04-27;50(4):50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The product of the ?-secretase processing of ephrinB2 regulates VE-cadherin complexes and angiogenesis
    Authors: NA Warren, G Voloudakis, Y Yoon, NK Robakis, A Georgakopo
    Cell. Mol. Life Sci., 2018-02-10;0(0):.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  13. EphrinB2 Activation Enhances Vascular Repair Mechanisms and Reduces Brain Swelling After Mild Cerebral Ischemia
    Authors: A Ghori, FB Freimann, M Nieminen-K, I Kremenetsk, K Gertz, M Endres, P Vajkoczy
    Arterioscler. Thromb. Vasc. Biol, 2017-03-02;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. CHMP5 controls bone turnover rates by dampening NF-kappaB activity in osteoclasts.
    Authors: Greenblatt M, Park K, Oh H, Kim J, Shin D, Lee J, Lee J, Singh A, Lee K, Hu D, Xiao C, Charles J, Penninger J, Lotinun S, Baron R, Ghosh S, Shim J
    J Exp Med, 2015-07-20;212(8):1283-301.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
    Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
    Nat Commun, 2015-03-26;6(0):6576.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells.
    Authors: White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, Srivastava D
    Stem Cells, 2013-01-01;31(1):92-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis.
    Authors: Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A
    Nature, 2010-05-05;465(7297):487-91.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Bioassay, ICC, Immunoprecipitation
  18. The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth.
    Authors: Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S
    Blood, 2009-07-13;114(9):1803-12.
    Species: Human
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  19. Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells.
    Authors: Narazaki G, Uosaki H, Teranishi M, Okita K, Kim B, Matsuoka S, Yamanaka S, Yamashita JK
    Circulation, 2008-07-14;118(5):498-506.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis.
    Authors: Masuda J, Usui R, Maru Y
    J. Biol. Chem., 2008-02-28;283(19):13148-55.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  21. Tyrosine phosphorylation sites in ephrinB2 are required for hippocampal long-term potentiation but not long-term depression.
    Authors: Bouzioukh F, Wilkinson GA, Adelmann G, Frotscher M, Stein V, Klein R
    J. Neurosci., 2007-10-17;27(42):11279-88.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway.
    Authors: Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J
    Biochem. Biophys. Res. Commun., 2004-10-08;323(1):17-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Inhibition of tumor growth and angiogenesis by soluble EphB4.
    Authors: Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG
    Neoplasia, 2004-05-01;6(3):248-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth.
    Authors: Noren NK, Lu M, Freeman AL, Koolpe M, Pasquale EB
    Proc. Natl. Acad. Sci. U.S.A., 2004-04-05;101(15):5583-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Flow regulates arterial-venous differentiation in the chick embryo yolk sac.
    Authors: le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A
    Development, 2003-12-17;131(2):361-75.
    Species: Avian - Quail, Chicken
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, In Vivo
  26. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
    Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
    J. Cell. Sci., 2003-05-06;116(0):2461-70.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Bioassay, ICC, IHC

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