Recombinant Mouse EGFR Fc Chimera Protein, CF

Catalog #: 1280-ER
2 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
1280-ER-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse EGFR Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Mouse EGFR Fc Chimera Protein binds to Recombinant Mouse EGF Protein (Catalog # 2028-EG) with an ED₅₀ of 0.500‑5.00 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse EGFR protein

Mouse EGFR (Leu25-Ser647) Accession # Q9EP98	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9EP98

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

95.9 kDa (monomer)

SDS-PAGE

118-130 kDa, reducing conditions

Product Datasheets

1280-ER

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1280-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44%-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

References

Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
Shilo, B.Z. (2005) Development 132:4017.
Avivi, A. et al. (1991) Oncogene 6:673.
Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
Reiter J.L. et al. (2001) Genomics 71:1.
Xu, Y.H. et al. (1984) Nature 309:806.
Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
Yarden, Y. et al. (1987) Biochemistry 26:1434.
Burgess, A.W. et al. (2003) Mol. Cell 12:541.
Lemmon, M.A. et al. (1997) EMBO J. 16:281.
Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
Sibilia, M. and E.F. Wagner (1995) Science 269:234.
Miettinen, P.J. et al. (1995) Nature 376:337.
Roskoski Jr. R. (2004) Biochem. Biophys. Res. Commun. 319:1.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Related Research Areas

Citations for Recombinant Mouse EGFR Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors
Authors: M Yun, DY Kim, JJ Lee, HS Kim, HS Kim, A Pyo, Y Ryu, TY Kim, JH Zheng, SW Yoo, H Hyun, G Oh, J Jeong, M Moon, JH Min, SY Kwon, JY Kim, E Chung, Y Hong, W Lee, HS Kim, JJ Min
Theranostics, 2017;7(10):2620-2633.
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Developmet
Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
Authors: Li N, Nguyen HH, Byrom M, Ellington AD
PLoS ONE, 2011-06-08;6(6):e20299.
Species: Human
Sample Types: RNA
Applications: Binding Assay