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Recombinant Mouse CXCL3/DCIP-1 Protein

Catalog #: 5568-CA
3 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
5568-CA-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5568-CA-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CXCL3/DCIP-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 25-100 ng/mL.
Source
E. coli-derived mouse CXCL3/GRO gamma/CINC-2/DCIP-1 protein
Ala28-Ser100
Accession #
AAI17017
N-terminal Sequence
Analysis
Ala28
Predicted Molecular Mass
7.9 kDa
SDS-PAGE
6.5 kDa, reducing conditions

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5568-CA (with carrier)

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5568-CA/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5568-CA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5568-CA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CXCL3 is also known as MIP-2 beta (macrophage inflammatory protein 2 beta), or DCIP-1 (dendritic cell inflammatory protein-1) in mouse, CINC2 (cytokine-induced neutrophil attractant 2) in rat, and GRO-gamma (growth-regulated oncogene gamma) in humans (1, 2). It is an 8 kDa proinflammatory member of the CXC subfamily of heparin-binding chemokines, also called alpha chemokines (1 - 4). The Glu-Leu-Arg (ELR) motif near the CXCL3 N-terminus confers angiogenic properties and distinguishes it from interferon-inducible ELR- CXC chemokines, which are angiostatic (4). ELR+ and ELR- chemokines use CXCR2 and CXCR3 receptors, respectively (3, 4). Mature mouse CXCL3 shares 88% and 57% amino acid (aa) sequence identity with rat and human CXCL3, respectively. Among mouse ELR+ chemokines, it shares 82% aa sequence identity with CXCL2/GRO-beta /MIP-2 and 34% - 58% with CXCL1/GRO-alpha /KC, CXCL5/ENA-78 and CXCL7/NAP-2. Due to their similar sequence and activity, CXCL2 and CXCL3 are sometimes referred to collectively as CXCL2/3, but are separate gene products (4 - 6). Mouse CXCL3 expression is induced in macrophages and early in maturation of DC by bacterial products such as lipopolysaccharides, and other inflammatory mediators (1, 7). It is chemotactic for CXCR2-expressing neutrophils, helping to recruit them to areas of inflammation (1, 7). ELR+ chemokines also elicit endothelial cell chemotaxis, stimulating angiogenesis and playing a role in tumor development (3, 4). ELR+ chemokines upregulated by ischemia play a role in ischemia-reperfusion injury (5, 6). A decoy receptor, DARC (Duffy antigen receptor for chemokines) competes with CXCR2 for ELR+ chemokine binding, thus downregulating their effect (8). Neutrophil influx may also be downregulated by MMP-12, which has been found to inactivate CXCL3 and other ELR+ chemokines by cleaving them at the ELR site (9).

References
  1. Nolan, K. F. et al. (2004) J. Immunol. 172:2201.
  2. Modi, W. S. and T. Yoshimura (1999) Mol. Biol. Evol. 16:180.
  3. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  4. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  5. Nesmelova, I. V. et al. (2008) J. Biol. Chem. 283:24155.
  6. Maheshwari, A. et al. (2004) Fetal Pediatr. Pathol. 23:145.
  7. Furuichi, K. et al. (2008) Front. Biosci. 13:4021.
  8. Takano, K. and H. Nakagawa (2001) Inflamm. Res. 50:503.
  9. Horton, L. W. et al. (2007) Cancer Res. 67:9791.
Entrez Gene IDs
2921 (Human); 330122 (Mouse); 171551 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3

Citations for Recombinant Mouse CXCL3/DCIP-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology
    Authors: Gullotta, GS;De Feo, D;Friebel, E;Semerano, A;Scotti, GM;Bergamaschi, A;Butti, E;Brambilla, E;Genchi, A;Capotondo, A;Gallizioli, M;Coviello, S;Piccoli, M;Vigo, T;Della Valle, P;Ronchi, P;Comi, G;D'Angelo, A;Maugeri, N;Roveri, L;Uccelli, A;Becher, B;Martino, G;Bacigaluppi, M;
    Nature immunology
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  2. Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors
    Authors: T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto
    Cancer Research, 2023-03-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Suppression of Medulloblastoma Lesions by Forced Migration of Preneoplastic Precursor Cells with Intracerebellar Administration of the Chemokine Cxcl3
    Front Pharmacol, 2016-12-16;7(0):484.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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