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Recombinant Mouse Complement Component C5a Protein

Catalog #: 2150-C5
39 Citations 1 Review Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
2150-C5-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2150-C5-025
Recombinant Mouse Complement Component C5a Protein Bioactivity
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Product Details
Citations (39)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Complement Component C5a Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 5-20 ng/mL.
Source
E. coli-derived mouse Complement Component C5a protein
Asn679-Arg755, with an N-terminal Met
Accession #
P06684
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
9.0 kDa

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2150-C5 (with carrier)

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2150-C5/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2150-C5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2150-C5/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Complement Component C5a Protein Bioactivity View Larger

Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is 5-20 ng/mL.

SDS-PAGE Recombinant Mouse Complement Component C5a Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Complement Component C5a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.

Background: Complement Component C5a

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

References
  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
  5. Okinaga, S. et al. (2003) Biochemistry 42:9406.
Entrez Gene IDs
727 (Human); 15139 (Mouse)
Alternate Names
C5; C5A; Complement Component C5a; HE

Citations for Recombinant Mouse Complement Component C5a Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Inflammatory mediators act at renal pericytes to elicit contraction of vasa recta and reduce pericyte density along the kidney medullary vascular network
    Authors: Lilley, RJ;Taylor, KD;Wildman, SSP;Peppiatt-Wildman, CM;
    Frontiers in physiology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. Investigation into the anti-airway inflammatory role of the PI3Kgamma inhibitor JN-PK1: An in vitro and in vivo study
    Authors: W Xiong, L Jia, J Liang, Y Cai, Y Chen, Y Nie, J Jin, J Zhu
    International immunopharmacology, 2022-08-11;111(0):109102.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Cell Culture
  3. Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells
    Authors: M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu
    Journal of Neuroinflammation, 2022-07-14;19(1):182.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions
    Authors: L Brandolini, M d'Angelo, R Novelli, V Castelli, C Giorgio, A Sirico, P Cocchiaro, F D'Egidio, E Benedetti, C Cristiano, A Bugatti, A Ruocco, PG Amendola, C Talarico, C Manelfi, D Iaconis, A Beccari, AU Quadros, TM Cunha, A Caruso, R Russo, A Cimini, A Aramini, M Allegretti
    Cell Death & Disease, 2022-05-25;13(5):500.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Heme catabolism by tumor-associated macrophages controls metastasis formation
    Authors: FM Consonni, A Bleve, MG Totaro, M Storto, P Kunderfran, A Termanini, F Pasqualini, C Alì, C Pandolfo, F Sgambellur, G Grazia, M Santinami, A Maurichi, M Milione, M Erreni, A Doni, M Fabbri, L Gribaldo, E Rulli, MP Soares, V Torri, R Mortarini, A Anichini, A Sica
    Nature Immunology, 2021-04-26;22(5):595-606.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis
    Authors: E van den Bo, B Ambrosy, M Horsthemke, S Walbaum, AC Bachg, N Wettschure, G Innamorati, TM Wilkie, PJ Hanley
    J. Biol. Chem., 2020-04-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  7. Two and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils
    Authors: AB Schmider, NC Bauer, H Sunwoo, MD Godin, GE Ellis, JT Lee, PA Nigrovic, RJ Soberman
    J. Biol. Chem., 2020-03-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. C3a elicits unique migratory responses in immature low-density neutrophils
    Authors: BE Hsu, J Roy, J Mouhanna, RF Rayes, L Ramsay, S Tabariès, MG Annis, IR Watson, JD Spicer, S Costantino, PM Siegel
    Oncogene, 2020-02-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Macrophage centripetal migration drives spontaneous healing process after spinal cord injury
    Authors: K Kobayakawa, Y Ohkawa, S Yoshizaki, T Tamaru, T Saito, K Kijima, K Yokota, M Hara, K Kubota, Y Matsumoto, K Harimaya, K Ozato, T Masuda, M Tsuda, T Tamura, K Inoue, VR Edgerton, Y Iwamoto, Y Nakashima, S Okada
    Sci Adv, 2019-05-15;5(5):eaav5086.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Phagocytosis of Apoptotic Cells Is Specifically Upregulated in ApoE4 Expressing Microglia in vitro
    Authors: C Muth, A Hartmann, D Sepulveda-, M Glatzel, S Krasemann
    Front Cell Neurosci, 2019-05-03;13(0):181.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  11. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4
    Authors: N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup
    Sci Rep, 2019-03-14;9(1):4515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis
    Authors: S Arandjelov, JSA Perry, CD Lucas, KK Penberthy, TH Kim, M Zhou, DA Rosen, TY Chuang, AM Bettina, LS Shankman, AH Cohen, A Gaultier, TP Conrads, M Kim, MR Elliott, KS Ravichandr
    Nat. Immunol., 2019-01-14;20(2):141-151.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Induction of Ocular Complement Activation by Inflammatory Stimuli and Intraocular Inhibition of Complement Factor D in Animal Models
    Authors: MA Crowley, O Delgado, A Will-Orreg, NM Buchanan, K Anderson, BD Jaffee, TP Dryja, SM Liao
    Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):940-951.
    Species: Mouse
    Sample Types: Plasma
    Applications: Bioassay
  14. Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration
    Authors: CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick
    Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):662-673.
    Species: Mouse
    Sample Types: Plasma
  15. Complement C5a Receptor 1 Exacerbates the Pathophysiology of N.�meningitidis Sepsis and Is a Potential Target for Disease Treatment
    Authors: JB Herrmann, M Muensterma, L Strobel, A Schubert-U, TM Woodruff, SD Gray-Owen, A Klos, KO Johswich
    MBio, 2018-01-23;9(1):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA (Standard)
  16. C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice
    Authors: SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt
    Eur. J. Immunol., 2018-01-15;0(0):.
    Applications: Bioassay
  17. Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg
    Authors: Y Wang, P Lai, X Chen, C He, X Huang, S Geng, C Luo, S Wu, W Ling, L Zhong, Z Lu, P Li, J Weng, X Du
    Sci Rep, 2017-06-15;7(1):3603.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing
    Authors: S Bergdolt, A Kovtun, Y Hägele, A Liedert, T Schinke, M Amling, M Huber-Lang, A Ignatius
    PLoS ONE, 2017-06-14;12(6):e0179512.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. The Ribosomal Protein S19 Suppresses Antitumor Immune Responses via the Complement C5a Receptor 1
    Authors: MM Markiewski, SK Vadrevu, SK Sharma, NK Chintala, S Ghouse, JH Cho, DP Fairlie, Y Paterson, A Astrinidis, M Karbownicz
    J. Immunol., 2017-02-22;198(7):2989-2999.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Sexual dimorphism of liver metastasis by murine pancreatic neuroendocrine tumors is affected by expression of complement C5
    Oncotarget, 2016-05-24;7(21):30585-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1
    J Neurosci, 2016-05-04;36(18):5055-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Blocking neutrophil diapedesis prevents hemorrhage during thrombocytopenia.
    Authors: Hillgruber C, Poppelmann B, Weishaupt C, Steingraber A, Wessel F, Berdel W, Gessner J, Ho-Tin-Noe B, Vestweber D, Goerge T
    J Exp Med, 2015-07-13;212(8):1255-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer.
    Authors: Yatime, Laure, Maasch, Christia, Hoehlig, Kai, Klussmann, Sven, Andersen, Gregers, Vater, Axel
    Nat Commun, 2015-04-22;6(0):6481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Complement 5a Enhances Hepatic Metastases of Colon Cancer via Monocyte Chemoattractant Protein-1-mediated Inflammatory Cell Infiltration.
    Authors: Piao C, Cai L, Qiu S, Jia L, Song W, Du J
    J Biol Chem, 2015-03-04;290(17):10667-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Distinct cellular sources of hepoxilin A3 and leukotriene B4 are used to coordinate bacterial-induced neutrophil transepithelial migration.
    Authors: Pazos M, Pirzai W, Yonker L, Morisseau C, Gronert K, Hurley B
    J Immunol, 2014-12-29;194(3):1304-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara.
    Authors: Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M
    J Immunol, 2014-12-29;194(3):1164-8.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  27. Cutting edge: critical role for C5aRs in the development of septic lymphopenia in mice.
    Authors: Grailer J, Fattahi F, Dick R, Zetoune F, Ward P
    J Immunol, 2014-12-24;194(3):868-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. High expression of C5L2 correlates with high proinflammatory cytokine expression in advanced human atherosclerotic plaques.
    Authors: Vijayan S, Asare Y, Grommes J, Soehnlein O, Lutgens E, Shagdarsuren G, Togtokh A, Jacobs M, Fischer J, Bernhagen J, Weber C, Schober A, Shagdarsuren E
    Am J Pathol, 2014-05-09;184(7):2123-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. IFN-gamma-producing NKT cells exacerbate sepsis by enhancing C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils.
    Authors: Kim J, Oh S, Ahn S, Chung D
    Eur J Immunol, 2014-05-02;44(7):2025-35.
    Applications: ELISA (Standard)
  30. C5L2 is required for C5a-triggered receptor internalization and ERK signaling.
    Authors: Hsu W, Yang F, Lin C, Hsieh S, Chen N
    Cell Signal, 2014-03-12;26(7):1409-19.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury.
    Authors: Bosmann, Markus, Grailer, Jamison, Ruemmler, Robert, Russkamp, Norman F, Zetoune, Firas S, Sarma, J Vidya, Standiford, Theodore, Ward, Peter A
    FASEB J, 2013-08-27;27(12):5010-21.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  32. A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis.
    Authors: Hoehlig, Kai, Maasch, Christia, Shushakova, Nelli, Buchner, Klaus, Huber-Lang, Markus, Purschke, Werner G, Vater, Axel, Klussmann, Sven
    Mol Ther, 2013-07-26;21(12):2236-46.
    Applications: Surface Plasmon Resonance
  33. The interaction between C5a and both C5aR and C5L2 receptors is required for production of G-CSF during acute inflammation.
    Authors: Bosmann M, Haggadone M, Zetoune F, Sarma J, Ward P
    Eur J Immunol, 2013-05-13;43(7):1907-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Local complement-targeted intervention in periodontitis: proof-of-concept using a C5a receptor (CD88) antagonist.
    Authors: Abe T, Hosur K, Hajishengallis E, Reis E, Ricklin D, Lambris J, Hajishengallis G
    J Immunol, 2012-10-22;189(11):5442-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  35. Opposing roles for complement component C5a in tumor progression and the tumor microenvironment.
    Authors: Gunn L, Ding C, Liu M, Ma Y, Qi C, Cai Y, Hu X, Aggarwal D, Zhang H, Yan J
    J Immunol, 2012-08-22;189(6):2985-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. The C5a Receptor on Mast Cells Is Critical for the Autoimmune Skin-blistering Disease Bullous Pemphigoid.
    Authors: Heimbach L, Li Z, Berkowitz P
    J. Biol. Chem., 2011-03-10;286(17):15003-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
    Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
    J. Immunol., 2010-12-13;186(2):869-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  38. C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a.
    Authors: Chen NJ, Mirtsos C, Suh D, Lu YC, Lin WJ, McKerlie C, Lee T, Baribault H, Tian H, Yeh WC
    Nature, 2007-02-25;446(7132):203-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  39. Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice.
    Authors: Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, Baumruker T, Billich A
    Immunol. Lett., 2007-01-30;109(1):56-63.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo

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Recombinant Mouse Complement Component C5a Protein
By Anonymous on 11/13/2019
Application: In vitro bioactivity in cell culture
In vitro bioactivity in cell culture Recombinant Mouse Complement Component C5a Protein 2150-C5