Home / CD40 Ligand/TNFSF5 / Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein

Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein

Catalog #: 8230-CL
20 Citations 4 Reviews Datasheet
Analyzed by SEC-MALS

Carrier Free

Catalog # Availability Size / Price Qty
8230-CL-050/CF

With Carrier

Catalog # Availability Size / Price Qty
8230-CL-050
Recombinant Mouse CD40 Ligand/TNFSF5 Protein SEC-MALS.
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Product Details
Citations (20)
FAQs
Reviews (4)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is 0.0700-0.350 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CD40 Ligand/TNFSF5 protein
HA
(YPYDVPDYA)
 GCN4-IZ GGGSGGGSGGGS Mouse CD40 Ligand/TNFSF5 
(Met112-Leu260)
Accession # P27548
N-terminus C-terminus
Accession #
P27548
N-terminal Sequence
Analysis
Tyr
Predicted Molecular Mass
22 kDa
SDS-PAGE
24-29 kDa, reducing conditions

Product Datasheets

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8230-CL (with carrier)

Product Datasheet

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8230-CL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8230-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in  sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8230-CL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) has a molecular weight (MW) of 66.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Background: CD40 Ligand/TNFSF5

CD40 Ligand, also known as TNFSF5, CD154, TRAP, or gp39, is a 33-39 kDa type II transmembrane glycoprotein member of the TNF superfamily (1, 2). Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region (1). The extracellular domain of mouse CD40 Ligand shares 75% and 93% amino acid (aa) sequence identity with the human and rat proteins, respectively. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (3). The 18 kDa soluble form (aa 112-260)  arises from proteolytic processing and retains the ability to bind and activate CD40 (4, 5). Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes (2). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (8, 9). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (10, 11).

References
  1. Armitage, R.J. et al. (1992) Nature 357:80.
  2. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
  3. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  4. Graf, D. et al. (1995) Eur. J. Immunol. 25:1749.
  5. Mazzei, G.J. et al. (1995) J. Biol. Chem. 270:7025.
  6. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  7. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  8. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  9. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  10. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  11. Hassan, G.S. et al. (2012) Immunobiology 217:521.
Entrez Gene IDs
959 (Human); 21947 (Mouse); 84349 (Rat)
Alternate Names
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

Citations for Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Targeting BMI-1 in B cells restores effective humoral immune responses and controls chronic viral infection
    Authors: A Di Pietro, J Polmear, L Cooper, T Damelang, T Hussain, L Hailes, K O'Donnell, V Udupa, T Mi, S Preston, A Shtewe, U Hershberg, SJ Turner, NL La Gruta, AW Chung, DM Tarlinton, CD Scharer, KL Good-Jacob
    Nature Immunology, 2021-11-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Histone Methyltransferase DOT1L Is Essential for Humoral Immune Responses
    Authors: L Kealy, A Di Pietro, L Hailes, S Scheer, L Dalit, JR Groom, C Zaph, KL Good-Jacob
    Cell Reports, 2020-12-15;33(11):108504.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells
    Authors: K Abdi, K Laky, K Padhan, C Petrovas, J Skinner, J Kabat, DW Dorward, J Brzostowsk, EO Long, G Trinchieri, R Varma
    J. Immunol., 2018-10-12;0(0):.
    Applications: Cell Culture
  4. Identification and characterisation of the CD40-ligand of Sigmodon hispidus
    Authors: MS Russell, A Muralidhar, L Larocque, J Cao, Y Deschambau, J Varga, SN Thulasi Ra, X Li
    PLoS ONE, 2018-07-27;13(7):e0199067.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA Developmet
  5. Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins
    Authors: S Trezise, A Karnowski, PL Fedele, S Mithraprab, Y Liao, K D'Costa, AJ Kueh, MP Hardy, CM Owczarek, MJ Herold, A Spencer, W Shi, SN Willis, SL Nutt, LM Corcoran
    Int J Mol Sci, 2018-07-24;19(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation
    Authors: BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss
    Nat Commun, 2018-05-15;9(1):1900.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Regulation of the DNA Repair Complex during Somatic Hypermutation and Class-Switch Recombination
    Authors: A Kumar, A Priya, T Ahmed, C Grundström, N Negi, T Grundström
    J. Immunol., 2018-05-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs
    Authors: CD Scharer, BG Barwick, M Guo, APR Bally, JM Boss
    Nat Commun, 2018-04-27;9(1):1698.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Zinc finger-IRF composite elements bound by Ikaros/IRF4 complexes function as gene repression in plasma cell
    Authors: K Ochiai, H Kondo, Y Okamura, H Shima, Y Kurokochi, K Kimura, R Funayama, T Nagashima, K Nakayama, K Yui, K Kinoshita, K Igarashi
    Blood Adv, 2018-04-24;2(8):883-894.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. T Follicular Helper Cell-Germinal Center B Cell Interaction Strength Regulates Entry into Plasma Cell or Recycling Germinal Center Cell Fate
    Authors: W Ise, K Fujii, K Shiroguchi, A Ito, K Kometani, K Takeda, E Kawakami, K Yamashita, K Suzuki, T Okada, T Kurosaki
    Immunity, 2018-04-17;48(4):702-715.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Bach2 Promotes B Cell Receptor-Induced Proliferation of B Lymphocytes and Represses Cyclin-Dependent Kinase Inhibitors
    Authors: Y Miura, M Morooka, N Sax, R Roychoudhu, A Itoh-Nakad, A Brydun, R Funayama, K Nakayama, S Satomi, M Matsumoto, K Igarashi, A Muto
    J. Immunol., 2018-03-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis
    Authors: S Cosenza, K Toupet, M Maumus, P Luz-Crawfo, O Blanc-Brud, C Jorgensen, D Noël
    Theranostics, 2018-02-03;8(5):1399-1410.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The Small Rho GTPase TC10 Modulates B Cell Immune Responses
    Authors: M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista
    J. Immunol., 2017-07-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo
    Authors: BC Lee, SG Lee, MK Choo, JH Kim, HM Lee, S Kim, DE Fomenko, HY Kim, JM Park, VN Gladyshev
    Sci Rep, 2017-07-11;7(1):5119.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. TLR3-Induced Maturation of Murine Dendritic Cells Regulates CTL Responses by Modulating PD-L1 Trafficking
    PLoS ONE, 2016-12-02;11(12):e0167057.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Regulated localization of an AID complex with E2A, PAX5 and IRF4 at the Igh locus
    Authors: Thomas Grundström
    Mol. Immunol., 2016-11-12;80(0):78-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Syk tyrosine kinase is critical for B cell antibody responses and memory B cell survival.
    Authors: Ackermann J, Nys J, Schweighoffer E, McCleary S, Smithers N, Tybulewicz V
    J Immunol, 2015-04-10;194(10):4650-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. IKK-induced NF-kappaB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen.
    Authors: Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, Tarlinton D, Tybulewicz V, Ley S
    J Exp Med, 2014-09-15;211(10):2085-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Roles of the kinase TAK1 in CD40-mediated effects on vascular oxidative stress and neointima formation after vascular injury.
    Authors: Song Z, Zhu X, Jin R, Wang C, Yan J, Zheng Q, Nanda A, Granger D, Li G
    PLoS ONE, 2014-07-22;9(7):e101671.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. The TACI receptor regulates T-cell-independent marginal zone B cell responses through innate activation-induced cell death.
    Authors: Figgett W, Fairfax K, Vincent F, Le Page M, Katik I, Deliyanti D, Quah P, Verma P, Grumont R, Gerondakis S, Hertzog P, O'Reilly L, Strasser A, Mackay F
    Immunity, 2013-09-05;39(3):573-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the purpose of the GCN4-IZ part of Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein) (Catalog # 8230-CL)?

    • CD40 Ligand naturally forms homotrimers, both as membrane bound and soluble forms. GCN4-IZ is a trimerization domain that promotes homotrimerization. We expect #8230-CL to include trimeric species; however, we do not have a structural specification for these products, relying instead on bioassay with a cross-linking antibody such as Anti-Hemagglutinin/HA Peptide antibody (Catalog # MAB060) to confirm consistency in bioactivity.

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Reviews for Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein

Average Rating: 4.8 (Based on 4 Reviews)

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Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
By Marina Theodorou on 05/20/2021
Application: CellProlif
Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
By Anonymous on 01/05/2021
Application: CellProlif
Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
By Lucia Sereni on 10/22/2020
Application: Cell Culture
Reason for Rating: I used this product in order to culture and expand murine B cells, along with the HA-Tag Antibody
Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
By Anonymous on 05/23/2016
Application: In vitro bioactivity in cell culture