Recombinant Mouse CD200R4 Protein, CF

Catalog #: 9275-CD Datasheet

Catalog #	Availability	Size / Price	Qty
9275-CD-050

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Recombinant Mouse CD200R4 Protein, CF Summary

R&D Systems NS0-derived Recombinant Mouse CD200R4 Protein (9275-CD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R4 is present at 0.5 μg/mL, Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) binds with a typical ED₅₀ of 0.8-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CD200 R4 protein
Thr26-Thr241, with a C-terminal 6-His tag

Accession #

Q6XJV4

N-terminal Sequence
Analysis

Thr26

Predicted Molecular Mass

24 kDa

SDS-PAGE

49-74 kDa, reducing conditions

Product Datasheets

9275-CD

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9275-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R4

CD200 R4, also known as CD200RLa, is a transmembrane protein in the immunoglobulin superfamily (1). Mature mouse CD200 R4 consists of a 216 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 8 aa cytoplasmic domain (2, 3). The ECD contains one Ig-like V-type domain and one Ig-like
C2-type domain. A positively charged lysine in the transmembrane likely mediates association with the signaling molecule DAP12 (2). CD200 R4 is expressed on monocytes, bone marrow derived macrophages and dendritic cells, NK cells, and resting mast cells. It is down-regulated following Fc epsilon RI-mediated mast cell activation (2). According to conflicting reports, CD200 R4 may or may not interact with CD200 (3, 4). R&D Systems in-house testing indicates that this recombinant mouse CD200 R4 binds to recombinant mouse CD200-Fc.

References