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Recombinant Mouse CCL11/Eotaxin Protein

Catalog #: 420-ME
15 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
420-ME-020/CF
420-ME-100/CF

With Carrier

Catalog # Availability Size / Price Qty
420-ME-020
420-ME-100
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Citations (15)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL11/Eotaxin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 1‑5 ng/mL.
Source
E. coli-derived mouse CCL11/Eotaxin protein
His24-Pro97
Accession #
P48298
N-terminal Sequence
Analysis
His24
Predicted Molecular Mass
8.4 kDa

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420-ME (with carrier)

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420-ME/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

420-ME

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

420-ME/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

References
  1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
  2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
  3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Entrez Gene IDs
6356 (Human); 20292 (Mouse)
Alternate Names
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Citations for Recombinant Mouse CCL11/Eotaxin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Reduced ccl11/eotaxin mediates the beneficial effects of environmental stimulation on the aged hippocampus
    Authors: G Scabia, G Testa, M Scali, S Del Turco, G Desiato, N Berardi, A Sale, M Matteoli, L Maffei, M Maffei, M Mainardi, “Train the
    Brain, Behavior, and Immunity, 2021-08-19;98(0):234-244.
    Species: Mouse
    Sample Types: In Vivo, Reference Standard
    Applications: Bioassay, Western Blot
  2. Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy
    Authors: F Boscolo Se, D Fox, A Sacco
    Cell Rep, 2019-01-15;26(3):689-701.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. CCL11, a novel mediator of inflammatory bone resorption
    Authors: E Kindstedt, CK Holm, R Sulniute, I Martinez-C, R Lundmark, P Lundberg
    Sci Rep, 2017-07-13;7(1):5334.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Rapid transport of CCL11 across the blood-brain barrier: regional variation and importance of blood cells.
    Authors: Erickson, Michelle, Morofuji, Yoichi, Owen, Joshua B, Banks, William
    J Pharmacol Exp Ther, 2014-04-04;349(3):497-507.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
    Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
    Nature, 2011-08-31;477(7362):90-4.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  6. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
    Authors: Nel HJ, Hams E, Saunders SP
    J. Immunol., 2011-03-11;186(8):4631-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
    Applications: ELISA (Standard)
  8. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Applications: ELISA (Standard)
  9. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Applications: ELISA (Standard)
  10. Discovery of CC chemokine receptor-3 (CCR3) antagonists with picomolar potency.
    Authors: De Lucca GV, Kim UT, Vargo BJ, Duncia JV, Santella JB, Gardner DS, Zheng C, Liauw A, Wang Z, Emmett G, Wacker DA, Welch PK, Covington M, Stowell NC, Wadman EA, Das AM, Davies P, Yeleswaram S, Graden DM, Solomon KA, Newton RC, Trainor GL, Decicco CP, Ko SS
    J. Med. Chem., 2005-03-24;48(6):2194-211.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003-02-01;170(3):1435-42.
    Applications: ELISA (Standard)
  13. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
    Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
    J. Leukoc. Biol., 2003-01-01;73(1):65-73.
    Applications: ELISA (Standard)
  14. Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding.
    Authors: Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP
    Eur. J. Pharmacol., 2002-12-05;456(1):1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002-04-01;160(4):1353-60.
    Applications: ELISA (Standard)

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