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Recombinant Mouse Artemin Protein

Catalog #: 1085-AR
10 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
1085-AR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1085-AR-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Artemin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-8 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera (Catalog # 2645-FR) at 1 µg/mL (100 µL/well) can bind Recombinant Mouse Artemin with an apparent KD <1 nM.
Source
E. coli-derived mouse Artemin protein
Ala112-Gly224
Accession #
Q9Z0L2.1
N-terminal Sequence
Analysis
Ala112
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12 kDa (monomer)

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1085-AR (with carrier)

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1085-AR/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1085-AR

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1085-AR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Artemin

Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similar to other TGF-beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component, cRET (receptor tyrosine kinase) (5-8). Artemin prefers to bind to GFR alpha -3 and activites the GFR alpha -3-RET. However, in the presence of RET, it can bind to GFR alpha -1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

References
  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Milbrandt, J. et al. (1998) Neuron 20:245.
  3. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J Biol Chem 272:33111.
  9. Nishino, J. et al. (1999) Neuron 23:725.
  10. Enomoto, H. et al. (2001) Development 128:3963.
  11. Andres, R. et al. (2001) Development 128:3685.
Entrez Gene IDs
9048 (Human); 11876 (Mouse)
Alternate Names
Artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin

Citations for Recombinant Mouse Artemin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. The neurotrophic factor artemin and its receptor GFR?3 mediate migraine-like pain via the ion channel TRPM8
    Authors: Yang, C;Wei, C;Alam, S;Chen, X;McKemy, DD;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. Endogenous inflammatory mediators produced by injury activate TRPV1 and TRPA1 nociceptors to induce sexually dimorphic cold pain that is dependent on TRPM8 and GFRalpha3
    Authors: C Yang, S Yamaki, T Jung, B Kim, R Huyhn, DD McKemy
    bioRxiv : the preprint server for biology, 2023-01-23;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Immunoglobulin somatic hypermutation in a defined biochemical system recapitulates affinity maturation and permits antibody optimization
    Authors: SL Jeong, H Zhang, S Yamaki, C Yang, DD McKemy, MR Lieber, P Pham, MF Goodman
    Nucleic Acids Research, 2022-11-11;0(0):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  4. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression
    Authors: Y Han, Q Liu, J Hou, Y Gu, Y Zhang, Z Chen, J Fan, W Zhou, S Qiu, Y Zhang, T Dong, N Li, Z Jiang, H Zhu, Q Zhang, Y Ma, L Zhang, Q Wang, Y Yu, N Li, X Cao
    Cell, 2018-03-29;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  5. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
    Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Inflammatory and neuropathic cold allodynia are selectively mediated by the neurotrophic factor receptor GFR?3
    Authors: EK Lippoldt, S Ongun, GK Kusaka, DD McKemy
    Proc Natl Acad Sci USA, 2016-04-05;113(16):4506-11.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Peripherally increased artemin is a key regulator of TRPA1/V1 expression in primary afferent neurons.
    Authors: Ikeda-Miyagawa Y, Kobayashi K, Yamanaka H, Okubo M, Wang S, Dai Y, Yagi H, Hirose M, Noguchi K
    Mol Pain, 2015-03-08;11(0):8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
    Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
    PLoS ONE, 2014-02-26;9(2):e89881.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice.
    Authors: Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ
    FEBS Lett., 2009-07-04;583(15):2479-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
    Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
    J. Neurosci., 2006-08-16;26(33):8588-99.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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