Recombinant Mouse Apolipoprotein H Protein, CF
Recombinant Mouse Apolipoprotein H Protein, CF Summary
Product Specifications
When Recombinant Mouse Apolipoprotein H/ApoH is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Mouse LDL R (Catalog # 2255-LD) that produces 50% of the optimal binding response is found to be approximately 0.05-0.25 μg/mL.
Gly20-Cys345, with a C-terminal 6-His tag
Analysis
Product Datasheets
6575-AH
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6575-AH
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Apolipoprotein H/ApoH
Apolipoprotein H (ApoH), also known as beta 2‑Glycoprotein I/ beta 2-GPI, is a 50 kDa variably glycosylated member of the complement control superfamily of proteins (1, 2). Mature mouse ApoH consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat (3, 4). Mature mouse ApoH shares 76% and 42% aa sequence identity with human and rat ApoH, respectively. Hepatocyte-derived ApoH binds directly to negatively charged phospholipids (5). It circulates as a component of lipoprotein particles and as a lipid-free serum protein (6). ApoH also associates with liposomes and apoptotic cell debris, thereby enabling their renal clearance via Megalin uptake (7, 8). Circulating levels of ApoH are postively correlated with triglyceride-rich lipoprotein (VLDL) components in type II diabetes (9). ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C (10, 11). ApoH can be cleaved by Plasmin at Lys317 ‑ Thr318, an action that is enhanced by heparin (12, 13). ApoH cleavage reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen (10, 12, 14). Cleaved ApoH also demonstrates antiangiogenic activity, whereas intact ApoH does not (14). The production of antibodies against ApoH is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages (15). ApoH binds to the surface antigen of Hepatitis B Virus and is associated with the development of HBV-induced hepatocellular carcinoma (6, 16).
- Crook, M.A. et al. (2010) Atherosclerosis 209:32.
- Miyakis, S. et al. (2004) Thromb. Res. 114:335.
- Nonaka, M. et al. (1992) Genomics 13:1082.
- Lozier, J. et al. (1984) Proc. Natl. Acad. Sci. 81:3640.
- Wurm, H. (1984) Int. J. Biochem. 16:511.
- Mehdi, H. et al. (1994) J. Virol. 68:2415.
- Chonn, A. et al. (1995) J. Biol. Chem. 270:25845.
- Moestrup, S.K. et al. (1998) J. Clin. Invest. 102:902.
- Castro, A. et al. (2010) Atherosclerosis 209:201.
- Shi, T. et al. (2004) Proc. Natl. Acad. Sci. 101:3939.
- Mori, T. et al. (1996) Thromb. Haemost. 75:49.
- Hunt, J. et al. (1993) Proc. Natl. Acad. Sci. 90:2141.
- Guerin, J. et al. (2002) J. Biol. Chem. 277:2644.
- Sakai, T. et al. (2007) Am. J. Pathol. 171:1659.
- Adams, M. (2008) Semin. Thromb. Haemost. 34:251.
- Jing, X. et al. (2010) J. Cancer Res. Clin. Oncol. 16:1671.
Citations for Recombinant Mouse Apolipoprotein H Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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T cells expressing the lupus susceptibility allele Pbx1d enhance autoimmunity and atherosclerosis in dyslipidemic mice
Authors: W Li, AS Elshikha, C Cornaby, X Teng, G Abboud, J Brown, X Zou, L Zeumer-Spa, B Robusto, SC Choi, K Fredenburg, A Major, L Morel
JCI Insight, 2020-06-04;5(11):.
Species: Mouse, Transgenic Mouse
Sample Types: Serum
Applications: ELISA Detection -
Hydroxychloroquine partially prevents endothelial dysfunction induced by anti-beta-2-GPI antibodies in an in vivo mouse model of antiphospholipid syndrome
Authors: G Urbanski, A Caillon, C Poli, G Kauffenste, MA Begorre, L Loufrani, D Henrion, C Belizna
PLoS ONE, 2018-11-06;13(11):e0206814.
Species: Mouse
Sample Types: Serum
Applications: ELISA (Capture) -
Nephropathy in Pparg-null mice highlights PPAR? systemic activities in metabolism and in the immune system
Authors: B Toffoli, F Gilardi, C Winkler, M Soderberg, L Kowalczuk, Y Arsenijevi, K Bamberg, O Bonny, B Desvergne
PLoS ONE, 2017-02-09;12(2):e0171474.
Species: Mouse
Sample Types: Plasma
Applications: Bioassay
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