Recombinant Mouse Angiopoietin-like 3 (aa 17-220), CF
Recombinant Mouse Angiopoietin-like 3 (aa 17-220), CF Summary
Product Specifications
Ser17-Thr206, with a C-terminal 6-His tag
Analysis
Product Datasheets
8344-AN
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8344-AN
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS,NaCl and CHAPS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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1 μg/lane of Recombinant Mouse Angiopoietin-like 3 (Catalog # 8344-AN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing both R and NR bands at 23-37 kDa.
Background: Angiopoietin-like Protein 3/ANGPTL3
Angiopoietin-like Protein 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1). Mature mouse ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (2). Within the N-terminal fragment, mouse ANGPTL3 shares 83% and 92% aa sequence identity with human and rat ANGPTL3, respectively. ANGPTL3 is expressed in the liver from early in development through adulthood (2, 3). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C-terminal fragments containing the coiled coil domain and fibrinogen-like domains, respectively (4, 5). The cleavage of ANGPTL3 by Furin and Proprotein Convertase 5/6 is enhanced by its interaction with the related ANGPTL8 (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (3, 4). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled coil domain (4). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo [Ono 41804]. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (4, 5, 8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (5, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with Integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (16).
- Santulli, G. (2014) Front. Endocrinol. (Lausanne) 5:4.
- Conklin, D. et al. (1999) Genomics 62:477.
- Ge, H. et al. (2005) J. Lipid Res. 46:1484.
- Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
- Koishi, R. et al. (2002) Nat. Genet. 30:151.
- Essalmani, R. et al. (2013) J. Biol. Chem. 288:26410.
- Quagliarini, F. et al. (2012) Proc. Natl. Acad. Sci. USA 109:19751.
- Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
- Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
- Koster, A. et al. (2005) Endocrinology 146:4943.
- Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
- Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
- Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
- Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23.
- Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
- Zhang, C.C. et al. (2006) Nat. Med. 12:240.
FAQs
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This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
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