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Recombinant Mouse Angiopoietin-1 Protein

Catalog #: 9936-AN
2 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
9936-AN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
9936-AN-025
Recombinant Mouse Angiopoietin-1 Protein Bioactivity
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Product Details
Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Angiopoietin-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is 10-50 ng/mL in the presence of 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived mouse Angiopoietin-1 protein
Mouse Angiopoietin-1
(Ser20-Leu261)
Accession # O08538		GGGSGGGSGGGSMouse Angiopoietin-1
(Arg277-Phe498)
Accession # O08538		HHHHHH
N-terminusC-terminus
Accession #
O08538
N-terminal Sequence
Analysis
Ser20
Structure / Form
Disulfide-linked Homooligomer
Predicted Molecular Mass
56 kDa
SDS-PAGE
59-75 kDa, reducing conditions

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9936-AN (with carrier)

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9936-AN/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9936-AN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

9936-AN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Angiopoietin-1 Protein Bioactivity View Larger

Recombinant Mouse Angiopoietin-1 inhibits serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-50 ng/mL in the presence of 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

SDS-PAGE Recombinant Mouse Angiopoietin-1 Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Angiopoietin‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 59-75 kDa and oligomer, respectively.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a N-terminal coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature Mouse Angiopoietin-1 shares 97% and 98% amino acid sequence identity with Human and rat Angiopoietin-1, respectively. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).

References
  1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.
  2. Suri, C. et al. (1996) Cell 87:1171.
  3. Davis, S. et al. (1996) Cell 87:1161.
  4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
  5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
  6. Suri, C. et al. (1998) Science 282:468.
  7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
  8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
  9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
  10. Jho, D. et al. (2005) Circ. Res. 96:1282.
  11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
  12. Kim, I. et al. (2001) Circ. Res. 89:477.
  13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
  14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
  15. Samuel, S.M. et al. (2010) Diabetes 59:51.
  16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.
Entrez Gene IDs
284 (Human); 11600 (Mouse)
Alternate Names
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

Citations for Recombinant Mouse Angiopoietin-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner
    Authors: R Luck, A Karakatsan, B Shah, G Schermann, H Adler, J Kupke, N Tisch, HW Jeong, MK Back, F Hetsch, A D'Errico, M De Palma, E Wiedtke, D Grimm, A Acker-Palm, J von Engelh, RH Adams, HG Augustin, C Ruiz de Al
    Cell Reports, 2021-08-17;36(7):109522.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  2. Neuromuscular Activity Induces Paracrine Signaling and Triggers Axonal Regrowth after Injury in Microfluidic Lab-On-Chip Devices
    Authors: J Sala-Jarqu, F Mesquida-V, M Badiola-Ma, J Samitier, A Hervera, JA Del Río
    Cells, 2020-01-27;9(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture

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