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Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF

Catalog #: 10739-CV Datasheet
Catalog # Availability Size / Price Qty
10739-CV-100
Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA with Recombinant Human DPPIV/CD26 (High Purity Dimer) (Catalog # 9168-SE).
Source
Human embryonic kidney cell, HEK293-derived mers-cov Spike protein
MERS-CoV Spike
(Tyr18-Lys1294)(Arg748Ser, Arg751Ser, Val1060Pro, Leu1061Pro)
Accession # YP_007188579.1		GCN4-IZ6-His tag
N-terminusC-terminus
Accession #
YP_007188579.1
N-terminal Sequence
Analysis
Tyr18
Predicted Molecular Mass
146 kDa
SDS-PAGE
145-175 kDa, under reducing conditions

Product Datasheets

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10739-CV

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10739-CV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Recombinant MERS-CoV Spike (GCN4-IZ) His-tag (Catalog # 10739-CV) binds Recombinant Human DPPIV/CD26 (High Purity Dimer) (9168-SE) in a functional ELISA.

SDS-PAGE View Larger

2 μg/lane of Recombinant MERS-CoV Spike (GCN4-IZ) His-tag (Catalog # 10739-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-175 kDa.

Background: Spike

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that also include SARS-CoV-1 and SARS-CoV-2. Coronaviruses are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). The MERS-CoV S protein shares 31% and 29% amino acid (aa) sequence identity with SARS-CoV S1 subunit and SARS-Cov2 S1 subunit, respectively. The low aa sequence homology is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). Unlike SARS-CoV and SARS-CoV2, which engage ACE-2 as their receptors for cell entry, MERS-CoV employs Dipeptidyl Peptidase 4 (DPP4; also known as CD26) as its functional receptor (4). Based on structural biology studies, the receptor binding domain (RBD) of MERS-CoV spike protein is located in the C-terminal region of S1 subunit and consists of a core subdomain and a receptor-binding subdomain (5, 6). The S1 subunit, especially the RBD region, was commonly targeted for vaccinations or antiviral therapy against MERS (7-9).

References
  1. Bermingham, A. et al. (2012) Euro Surveill. 17:20290.
  2. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Lu, G. et al. (2013) Nature 500:227.
  6. Wang, N. et al. (2013) Cell. Res. 23:986.
  7. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  8. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  9. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.
Long Name
Spike Protein
Entrez Gene IDs
918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)
Alternate Names
2019-nCoV S Protein; 2019-nCoV Spike; COVID-19 Spike; E2; Human coronavirus spike glycoprotein; Peplomer protein; S glycoprotein; S Protein; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; SARS-CoV-2; Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein; Spike glycoprotein; Spike; surface glycoprotein

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